Linked Life Data

19838772

Source:http://linkedlifedata.com/resource/pubmed/id/19838772

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-2-11
pubmed:abstractText
Polyethylenimine (PEI) is one of the most widely investigated cationic polymers for gene delivery. However, PEI/DNA complexes are unstable and tend to aggregate. PEGylation was used to improve the stability. The stability of polymer/DNA complexes was investigated including complexation stability, aggregation stability, sedimentation stability, and nuclease stability. PEI25K/DNA complexes were liable to aggregate to large particles (500-700 nm). The aggregation was proved to be induced by phosphate anion. In the medium without phosphate anion, aggregation was prevented by electrostatic repulsion. Owing to more efficient steric repulsion, PEG2 and PEG5K excelled PEG750 in facilitating copolymers to form stable small polyplexes (below 100 nm) without aggregation regardless of phosphate anion. The steric repulsion predominated over electrostatic repulsion in stabilization.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1573-4838
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
597-607
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Stability of poly(ethylene glycol)-graft-polyethylenimine copolymer/DNA complexes: influences of PEG molecular weight and PEGylation degree.
pubmed:affiliation
School of Pharmaceutical Science, Sun Yat-Sen University, Guangzhou, 510006, China. luoxin703@163.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't