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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1991-1-17
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pubmed:abstractText |
Using a simple fluorescence test, we show that commercially prepared colloidal gold complexes with goat second antibodies often contain free active antibody. Because such antibodies will compete with antibody-colloidal gold particles for antigen binding sites, labeling intensity at the ultrastructural level must necessarily be submaximal to an unknown degree with such preparations. A survey of five preparations suggests that the problem may be widespread. We recommend that a test of the sort described be incorporated routinely into protocols with all colloidal gold products.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-1554
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
37-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1983872-Animals,
pubmed-meshheading:1983872-Antibodies,
pubmed-meshheading:1983872-Antibodies, Anti-Idiotypic,
pubmed-meshheading:1983872-Colloids,
pubmed-meshheading:1983872-Fluorescent Antibody Technique,
pubmed-meshheading:1983872-Gold,
pubmed-meshheading:1983872-Immunoglobulin G,
pubmed-meshheading:1983872-Immunohistochemistry,
pubmed-meshheading:1983872-Indicators and Reagents,
pubmed-meshheading:1983872-Mice,
pubmed-meshheading:1983872-Rats
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pubmed:year |
1991
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pubmed:articleTitle |
Commercial preparations of colloidal gold-antibody complexes frequently contain free active antibody.
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pubmed:affiliation |
Department of Physiology, University of North Carolina, Chapel Hill 27599.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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