Linked Life Data

19835951

Source:http://linkedlifedata.com/resource/pubmed/id/19835951

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2009-12-7
pubmed:abstractText
A novel pathogenicity island, SPI-18, carries the taiA-hlyE operon, encoding virulence factors in Salmonella Typhi. To determine the effects of certain environmental conditions on the expression of these genes, beta-galactosidase assays, RT-PCR reactions, western blot analyses and measurement of hemolytic activity were performed. The conditions studied are those likely found by S. Typhi during infection in the human host. We found RpoS-dependent transcriptional upregulation in low pH and high osmolarity for both genes. Our results show that oxygen depletion apparently did not affect transcription of the taiA-hlyE operon. On the other hand, the transcriptional regulator Crp, previously described as an activator of hlyE transcription in Escherichia coli, is involved in transcriptional repression of hlyE in S. Typhi. Moreover, addition of glucose to the growth medium results in decreasing the hlyE mRNA, suggesting that there is another factor related to catabolite repression different from Crp and involved in downregulation of hlyE in S. Typhi.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1769-7123
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
160
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
800-8
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
RpoS- and Crp-dependent transcriptional control of Salmonella Typhi taiA and hlyE genes: role of environmental conditions.
pubmed:affiliation
Laboratorio de Microbiología, Facultad de Ciencias Biológicas y Facultad de Medicina, Universidad Andres Bello, República 217, Santiago de Chile, Chile. juan.fuentesa@uandresbello.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't