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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2010-2-15
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pubmed:abstractText |
A recombinant human antibody expressed in corn was purified using aqueous two-phase extraction. The antibody was an immunoglobulin G fully unglycosylated. Using systems of different compositions and/or pHs in each of one or two partitioning stages followed by one more stage in which the antibody was precipitated at the liquid/liquid interface facilitated the removal of different impurities in each stage. The best system yields a product 72% pure (22-fold purification) with a yield of 49%. The optimum extraction was done in two partitioning stages followed by an interfacial precipitation stage using poly(ethylene)glycol/potassium phosphate systems. NaCl was added to the first stage to eliminate large molecular weight impurities. The pH in the first stage was kept at 6 but a pH of 8 was used in the second stage and in the precipitation stage.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1520-6033
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
159-67
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pubmed:dateRevised |
2011-5-26
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pubmed:meshHeading |
pubmed-meshheading:19834982-Chromatography, Gel,
pubmed-meshheading:19834982-Chromatography, High Pressure Liquid,
pubmed-meshheading:19834982-Electrophoresis, Capillary,
pubmed-meshheading:19834982-Humans,
pubmed-meshheading:19834982-Hydrogen-Ion Concentration,
pubmed-meshheading:19834982-Immunoglobulin G,
pubmed-meshheading:19834982-Molecular Weight,
pubmed-meshheading:19834982-Phosphates,
pubmed-meshheading:19834982-Polyethylene Glycols,
pubmed-meshheading:19834982-Potassium Compounds,
pubmed-meshheading:19834982-Recombinant Proteins,
pubmed-meshheading:19834982-Sodium Chloride,
pubmed-meshheading:19834982-Water,
pubmed-meshheading:19834982-Zea mays
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pubmed:articleTitle |
Purification of human antibodies from transgenic corn using aqueous two-phase systems.
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pubmed:affiliation |
Dept. of Chemical and Biological Engineering, Missouri University of Science and Technology, Rolla, MO 65409, USA.
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pubmed:publicationType |
Journal Article
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