Linked Life Data

19833716

Source:http://linkedlifedata.com/resource/pubmed/id/19833716

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2009-11-25
pubmed:abstractText
Well-controlled trophoblast invasion into uterine decidua is a critical process for the normal development of placenta, which is tightly regulated by various factors produced within the trophoblast-endometrial microenvironment. CXCL14 is involved in tumor growth and metastasis, and its expression in placenta is temporally regulated during pregnancy. However, the role of CXCL14 in trophoblast function during human pregnancy is not clear. In this study, by using RT-PCR through human pregnancy, we found that CXCL14 was selectively expressed at early but not late pregnancy. Immunostaining revealed that CXCL14 proteins were strongly expressed in villous cytotrophoblasts and moderately in decidualized stromal cells but very weakly in syncytiotrophoblasts and extravillous trophoblasts. The effect of CXCL14 on trophoblast invasion were examined by using human villous explants cultured on Matrigel and further proved by invasion and migration assay of primary trophoblast cells and trophoblast cell line HTR-8/SVneo. Our data showed that CXCL14 significantly inhibited outgrowth of villous explant in vitro; this effect is due to suppression of trophoblast invasion and migration through regulating matrix metalloproteinases activities, whereas the trophoblast proliferation was not affected. Moreover, because a receptor for CXCL14 has not been identified, we performed further cell-specific CXCL14 binding activities with regard to different cell types within the maternal-fetal interface. Our data revealed that CXCL14 could specifically bind to trophoblast cells but not decidual cells from the maternal-fetal interface. These results suggest that CXCL14 plays an important role in regulating trophoblast invasion through an autocrine/paracrine manner during early pregnancy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1945-7170
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
150
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5596-605
pubmed:meshHeading
pubmed-meshheading:19833716-Cell Line, pubmed-meshheading:19833716-Cell Movement, pubmed-meshheading:19833716-Cell Proliferation, pubmed-meshheading:19833716-Cell Survival, pubmed-meshheading:19833716-Cells, Cultured, pubmed-meshheading:19833716-Chemokines, CXC, pubmed-meshheading:19833716-Decidua, pubmed-meshheading:19833716-Female, pubmed-meshheading:19833716-Gelatinases, pubmed-meshheading:19833716-Gene Expression Regulation, Developmental, pubmed-meshheading:19833716-Humans, pubmed-meshheading:19833716-Immunohistochemistry, pubmed-meshheading:19833716-Matrix Metalloproteinase 2, pubmed-meshheading:19833716-Matrix Metalloproteinase 9, pubmed-meshheading:19833716-Placenta, pubmed-meshheading:19833716-Pregnancy, pubmed-meshheading:19833716-Protein Binding, pubmed-meshheading:19833716-Recombinant Proteins, pubmed-meshheading:19833716-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:19833716-Tissue Culture Techniques, pubmed-meshheading:19833716-Tissue Inhibitor of Metalloproteinase-1, pubmed-meshheading:19833716-Tissue Inhibitor of Metalloproteinase-2, pubmed-meshheading:19833716-Trophoblasts
pubmed:year
2009
pubmed:articleTitle
The cytokine gene CXCL14 restricts human trophoblast cell invasion by suppressing gelatinase activity.
pubmed:affiliation
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't