Linked Life Data

19824668

Source:http://linkedlifedata.com/resource/pubmed/id/19824668

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2009-11-25
pubmed:abstractText
Successful delivery of labile vaccine antigens, such as peptides and proteins, to stimulate CD4 and CD8 T cell immunity could improve vaccine strategies against chronic infections such as HIV and Hepatitis C. Layer-by-layer (LbL)-assembled nanoengineered hydrogel capsules represent a novel and promising technology for the protection and delivery of labile vaccine candidates to antigen-presenting cells (APCs). Here we report on the in vitro and in vivo immunostimulatory capabilities of LbL-assembled disulfide cross-linked poly(methacrylic acid) (PMA(SH)) hydrogel capsules as a delivery strategy for protein and peptide vaccines using robust transgenic mice models and ovalbumin (OVA) as a model vaccine. We demonstrate that OVA protein as well as multiple OVA peptides can be successfully encapsulated within nanoengineered PMA(SH) hydrogel capsules. OVA-containing PMA(SH) capsules are internalized by mouse APCs, resulting in presentation of OVA epitopes and subsequent activation of OVA-specific CD4 and CD8 T cells in vitro. OVA-specific CD4 and CD8 T cells are also activated to proliferate in vivo following intravenous vaccination of mice with OVA protein- and OVA peptide-loaded PMA(SH) hydrogel capsules. Furthermore, we show that OVA encapsulated within the PMA(SH) capsules resulted in at least 6-fold greater proliferation of OVA-specific CD8 T cells and 70-fold greater proliferation of OVA-specific CD4 T cells in vivo compared to the equivalent amount of OVA protein administered alone. These results highlight the potential of nanoengineered hydrogel capsules for vaccine delivery.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1936-086X
pubmed:author
pubmed:issnType
Electronic
pubmed:day
24
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3391-400
pubmed:meshHeading
pubmed-meshheading:19824668-Amino Acid Sequence, pubmed-meshheading:19824668-Animals, pubmed-meshheading:19824668-Antigen Presentation, pubmed-meshheading:19824668-Antigen-Presenting Cells, pubmed-meshheading:19824668-Antigens, CD4, pubmed-meshheading:19824668-Antigens, CD8, pubmed-meshheading:19824668-Biological Transport, pubmed-meshheading:19824668-Capsules, pubmed-meshheading:19824668-Cell Proliferation, pubmed-meshheading:19824668-Drug Carriers, pubmed-meshheading:19824668-Drug Delivery Systems, pubmed-meshheading:19824668-Hydrogels, pubmed-meshheading:19824668-Immunization, pubmed-meshheading:19824668-Intracellular Space, pubmed-meshheading:19824668-Mice, pubmed-meshheading:19824668-Mice, Transgenic, pubmed-meshheading:19824668-Molecular Sequence Data, pubmed-meshheading:19824668-Nanotechnology, pubmed-meshheading:19824668-Ovalbumin, pubmed-meshheading:19824668-Peptide Fragments, pubmed-meshheading:19824668-Polymethacrylic Acids, pubmed-meshheading:19824668-Receptors, Antigen, T-Cell, pubmed-meshheading:19824668-T-Lymphocytes, pubmed-meshheading:19824668-Vaccines
pubmed:year
2009
pubmed:articleTitle
A protective vaccine delivery system for in vivo T cell stimulation using nanoengineered polymer hydrogel capsules.
pubmed:affiliation
Department of Microbiology & Immunology, The University of Melbourne, Parkville, Victoria, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't