Linked Life Data

1982130

Source:http://linkedlifedata.com/resource/pubmed/id/1982130

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1991-5-10
pubmed:abstractText
The opsonizing effect of normal cerebrospinal fluid (CSF) on Staphylococcus aureus has been demonstrated through phagocytic assay. Radiolabelled S. aureus was opsonized using various CSF preparations and then ingested by neutrophils. Uptake of S. aureus by neutrophils was 31 +/- 12% for untreated CSF, 28 +/- 14% for ethylene-glycol-bis(beta-aminoethyl ether) -N,N-tetraacetic acid(EGTA) chelation, 18 +/- 5% for heat-treated (50 degrees C), 14 +/- 8% for heat-inactivated (56 degrees C), 11 +/- 6% for S. aureus-absorbed, and 5 +/- 4% for heat-inactivated and S. aureus-absorbed, respectively. Indirect immunofluorescence antibody assay revealed that IgG was the class of immunoglobulins involved in the opsonic activity of CSF, and complement 3 (C3) deposition was weakly detected in S. aureus. These results suggest that both heat-labile (complement) and heat-stable (antibody) factors are crucial for the opsonization of S. aureus by normal CSF. We conclude that activation of the complement by S. aureus in normal CSF is through an alternative pathway and is enhanced by the presence of a specific antibody (IgG).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0929-6646
pubmed:author
pubmed:issnType
Print
pubmed:volume
89
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
977-81
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Opsonizing effect of normal cerebrospinal fluid on Staphylococcus aureus.
pubmed:affiliation
Department of Parasitology, National Defense Medical Center, Taipei, Taiwan, R.O.C.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't