Source:http://linkedlifedata.com/resource/pubmed/id/19820690
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
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pubmed:dateCreated |
2009-11-30
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pubmed:abstractText |
The discrimination between well-differentiated liposarcomas/atypical lipomatous tumors and lipomas can be diagnostically challenging at the histological level. However, cytogenetic identification of ring and giant rod chromosomes supports the diagnosis of well-differentiated liposarcoma/atypical lipomatous tumor. These abnormal chromosomes are mainly composed of amplified genomic sequences derived from chromosome 12q13-15, and contain several genes, including MDM2, CDK4 (SAS), TSPAN31, HMGA2, and others. MDM2 is consistently amplified in well-differentiated liposarcomas/atypical lipomatous tumors, and up to 25% in other sarcomas. As part of a large genomic study of lipomatous neoplasms, we initially found CPM to be consistently amplified in well-differentiated liposarcomas/atypical lipomatous tumors. To further explore this initial finding, we investigated the copy number status of MDM2 and CPM by fluorescent in situ hybridization (FISH) on a series of 138 tumors and 17 normal tissues, including 32 well-differentiated liposarcoma/atypical lipomatous tumors, 63 lipomas, 11 pleomorphic lipomas, 2 lipoblastomas, 30 other tumors and 17 normal fat samples. All 32 well-differentiated liposarcoma/atypical lipomatous tumors showed amplification of MDM2 and CPM, usually >20 copies per cell. The other tumors lacked MDM2 and/or CPM amplification. Chromogenic in situ hybridization confirmed the above results on a subset of these tumors (n=27). These findings suggest that identification of CPM amplification could be used as an alternative diagnostic tool for the diagnosis of well-differentiated liposarcoma/atypical lipomatous tumors.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/GPI-Linked Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/MDM2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Metalloendopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-mdm2,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological,
http://linkedlifedata.com/resource/pubmed/chemical/carboxypeptidase M
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1530-0285
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pubmed:author |
pubmed-author:AsmannYan WYW,
pubmed-author:Erickson-JohnsonMichele RMR,
pubmed-author:HulshizerRachael LRL,
pubmed-author:JacobEapen KEK,
pubmed-author:KingAllison AAA,
pubmed-author:LloydRicardo VRV,
pubmed-author:OliveiraAndre MAM,
pubmed-author:RothChristopher WCW,
pubmed-author:SeysAmber RAR,
pubmed-author:WangXiaokeX
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pubmed:issnType |
Electronic
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1541-7
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:19820690-Cell Differentiation,
pubmed-meshheading:19820690-Comparative Genomic Hybridization,
pubmed-meshheading:19820690-Diagnosis, Differential,
pubmed-meshheading:19820690-GPI-Linked Proteins,
pubmed-meshheading:19820690-Gene Amplification,
pubmed-meshheading:19820690-Gene Dosage,
pubmed-meshheading:19820690-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:19820690-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:19820690-Genetic Testing,
pubmed-meshheading:19820690-Humans,
pubmed-meshheading:19820690-In Situ Hybridization, Fluorescence,
pubmed-meshheading:19820690-Lipoma,
pubmed-meshheading:19820690-Liposarcoma,
pubmed-meshheading:19820690-Metalloendopeptidases,
pubmed-meshheading:19820690-Predictive Value of Tests,
pubmed-meshheading:19820690-Proto-Oncogene Proteins c-mdm2,
pubmed-meshheading:19820690-Tumor Markers, Biological
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pubmed:year |
2009
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pubmed:articleTitle |
Carboxypeptidase M: a biomarker for the discrimination of well-differentiated liposarcoma from lipoma.
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pubmed:affiliation |
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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