Linked Life Data

19818595

Source:http://linkedlifedata.com/resource/pubmed/id/19818595

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2010-2-3
pubmed:abstractText
The cell cycle plays a crucial role in many cellular physiological processes and has drawn an increasing interest in past decades. In the current study, we have developed a bioelectronic chip-based system capable of performing real-time dynamic analysis of the cell cycle in live cells via non-invasive cellular impedance sensing. The cells were cultured on the cellular impedance sensing chip comprising microfabricated interdigitated electrode structures. HeLa cells were synchronized with double thymidine block and the cellular impedances were monitored in a time resolution of minutes during the whole 5 days' experiment. Our results show that real-time impedance sensing can clearly mirror the progression of the cell cycle-in G(1) phase and S phase, the cellular impedance increased with time, while in G(2) phase and M phase, the cellular impedance decreased. Correspondingly, the time-course impedance curves for the synchronized cells have marked "M-valleys" corresponding to the periods of mitosis of the cells. The cell cycle information revealed by the cellular impedance data was confirmed using flow cytometry and microscopy. This paper presents the first step towards in situ and label-free monitoring of the cell cycle of mammalian cells on chip in a real-time, non-invasive manner.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1873-4235
pubmed:author
pubmed:copyrightInfo
Copyright 2009 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
990-5
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Real-time, label-free monitoring of the cell cycle with a cellular impedance sensing chip.
pubmed:affiliation
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies