Source:http://linkedlifedata.com/resource/pubmed/id/19818317
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
2009-10-12
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pubmed:abstractText |
Bacillus sphaericus AKU 229 was found to produce an acetaldehyde-tolerant and phosphorylated compound-tolerant phosphopentomutase useful for enzymatic 2'-deoxyribonucleoside production. The gene encoding the phosphopentomutase was cloned and expressed in Escherichia coli. The E. coli expressing B. sphaericus phosphopentomutase was an excellent catalyst as to production of 2'-deoxyribonucleoside in the presence of acetaldehyde and phosphorylated compounds such as fructose 1,6-diphosphate, and d-glyceraldehyde 3-phosphate, which are derived from glucose through glycolysis with yeast cells, and exist abundantly in the practical reaction mixture for enzymatic 2'-deoxyribonucleoside production.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1876-4347
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
75-82
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pubmed:meshHeading |
pubmed-meshheading:19818317-Acetaldehyde,
pubmed-meshheading:19818317-Amino Acid Sequence,
pubmed-meshheading:19818317-Bacillus,
pubmed-meshheading:19818317-Biocatalysis,
pubmed-meshheading:19818317-Biotechnology,
pubmed-meshheading:19818317-Cloning, Molecular,
pubmed-meshheading:19818317-Deoxyribonucleosides,
pubmed-meshheading:19818317-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:19818317-Escherichia coli,
pubmed-meshheading:19818317-Metabolic Networks and Pathways,
pubmed-meshheading:19818317-Molecular Sequence Data,
pubmed-meshheading:19818317-Phosphorylation,
pubmed-meshheading:19818317-Phosphotransferases,
pubmed-meshheading:19818317-Sequence Alignment,
pubmed-meshheading:19818317-Transformation, Genetic
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pubmed:year |
2009
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pubmed:articleTitle |
Screening and characterization of a phosphopentomutase useful for enzymatic production of 2'-deoxyribonucleoside.
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pubmed:affiliation |
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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