|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2009-12-17
|
|
pubmed:abstractText |
In this study, we demonstrate that human neuroblastoma SH-SY5Y cells transfected with human tyrosine hydroxylase isoform 1 (SH + TH cells) were substantially more resistant to cell death induced by hydrogen peroxide and 6-hydroxydopamine when compared to wild-type SH-SY5Y cells (SH cells). SH + TH cells exhibit increased levels of dopamine (DA) compared to SH cells. Incubation with hydrogen peroxide or 6-hydroxydopamine (10-100microM) for 24 h caused a significant reduction in cell viability and increased apoptosis in both cell types. However, these effects were significantly reduced in the SH + TH cells when compared to the SH cells. The SH + TH cells showed an improved ability to detoxify peroxide, which correlated with an increase in glutathione peroxidase and glutathione reductase activities, while catalase activity was unchanged. Our data suggest that a preconditioning-like mechanism linked to higher DA levels increased the resistance of SH + TH cells against oxidative insults, which is at least in part related to an augmentation in the activity of glutathione-related antioxidant enzymes.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
1096-0929
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
113
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
150-7
|
|
pubmed:dateRevised |
2010-9-17
|
|
pubmed:meshHeading |
pubmed-meshheading:19815691-Apoptosis,
pubmed-meshheading:19815691-Carmustine,
pubmed-meshheading:19815691-Catalase,
pubmed-meshheading:19815691-Cell Line, Tumor,
pubmed-meshheading:19815691-Cell Survival,
pubmed-meshheading:19815691-Dopamine,
pubmed-meshheading:19815691-Dose-Response Relationship, Drug,
pubmed-meshheading:19815691-Enzyme Inhibitors,
pubmed-meshheading:19815691-Glutathione Peroxidase,
pubmed-meshheading:19815691-Glutathione Reductase,
pubmed-meshheading:19815691-Humans,
pubmed-meshheading:19815691-Hydrogen Peroxide,
pubmed-meshheading:19815691-Neuroblastoma,
pubmed-meshheading:19815691-Oxidative Stress,
pubmed-meshheading:19815691-Oxidopamine,
pubmed-meshheading:19815691-Thiomalates,
pubmed-meshheading:19815691-Transfection,
pubmed-meshheading:19815691-Tyrosine 3-Monooxygenase
|
|
pubmed:year |
2010
|
|
pubmed:articleTitle |
Expression of tyrosine hydroxylase increases the resistance of human neuroblastoma cells to oxidative insults.
|
|
pubmed:affiliation |
Centro de Ciências Rurais, Universidade Federal do Pampa, São Gabriel, Rio Grande do Sul 97300-000, Brazil. jefersonbio@hotmail.com
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
