Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1991-4-11
pubmed:abstractText
A new chromogen, Indophane Blue (IB) was tested for use in HRP-tract tracing and double-label immunocytochemistry. Although TMB was slightly more sensitive and delineated individual labeled fibers better than IB in tract-tracing experiments, there were two advantages to using IB over TMB. First, the IB reaction could be performed at a pH of 6.0, rather than the pH of 3.3 required for TMB, resulting in much better preservation of tissue. Second, there was no crystalline artifact as has been observed with TMB. In the double-label immunocytochemistry experiments, the combination of diaminobenzidine for the first chromogen and IB as the second, provided excellent contrast.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0361-9230
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
393-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
A new chromogen for use in HRP-tract tracing and double-label immunocytochemistry.
pubmed:affiliation
Department of Anatomy and Cell Biology, University of Cincinnati College of Medicine, OH 45267-0521.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.