Linked Life Data

19812258

Source:http://linkedlifedata.com/resource/pubmed/id/19812258

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2009-12-1
pubmed:abstractText
Schistosomiasis japonica is an endemic, zoonotic disease of major public health importance in China. Control programs combining chemotherapy and snail killing have not been able to block transmission of infection in lakes and marsh regions. Vaccination is needed as a complementary approach to the ongoing control programs. In the present study, we wanted to determine if the efficacies of DNA vaccines encoding the 23-kDa tetraspanin membrane protein (SjC23), triose phosphate isomerase (SjCTPI), and sixfold-repeated genes of the complementarity determining region 3 (CDR3) in the H chain of NP30 could be enhanced by boosting via electroporation in vivo and/or with cocktail protein vaccines. Mice vaccinated with cocktail DNA vaccines showed a significant worm reduction of 32.88% (P < 0.01) and egg reduction of 36.20% (P < 0.01). Vaccine efficacy was enhanced when animals were boosted with cocktail protein vaccines; adult worm and liver egg burdens were reduced 45.35% and 48.54%, respectively. Nearly identical results were obtained in mice boosted by electroporation in vivo, with adult worm and egg burdens reduced by 45.00% and 50.88%, respectively. The addition of a protein vaccine boost to this regimen further elevated efficacy to approximately 60% for adult worm burden and greater than 60% for liver egg reduction. The levels of interleukin-2, gamma interferon, and the ratios of immunoglobulin G2a (IgG2a)/IgG1 clearly showed that cocktail DNA vaccines induced CD4(+) Th1-type responses. Boosting via either electroporation or with recombinant proteins significantly increased associated immune responses over those seen in mice vaccinated solely with DNA vaccines. Thus, schistosome DNA vaccine efficacy was significantly enhanced via boosting by electroporation in vivo and/or cocktail protein vaccines.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-10200250, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-10476210, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-10719119, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-10779767, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-10996119, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-11292639, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-12020899, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-12049454, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-12076047, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-12213407, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-12236413, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-14506212, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-14533200, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-14670333, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-15115073, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-15385516, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-15519710, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-15557616, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-15879128, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-16154246, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-16393355, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-16790382, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-1690918, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-17669562, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-18524429, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-7722323, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-8063386, http://linkedlifedata.com/resource/pubmed/commentcorrection/19812258-9743122
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1556-679X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1796-803
pubmed:dateRevised
2011-9-26
pubmed:meshHeading
pubmed-meshheading:19812258-Animals, pubmed-meshheading:19812258-Antibodies, Helminth, pubmed-meshheading:19812258-Antigens, Helminth, pubmed-meshheading:19812258-Electroporation, pubmed-meshheading:19812258-Female, pubmed-meshheading:19812258-Helminth Proteins, pubmed-meshheading:19812258-Immunization, Secondary, pubmed-meshheading:19812258-Immunoglobulin G, pubmed-meshheading:19812258-Interferon-gamma, pubmed-meshheading:19812258-Interleukin-2, pubmed-meshheading:19812258-Membrane Proteins, pubmed-meshheading:19812258-Mice, pubmed-meshheading:19812258-Mice, Inbred BALB C, pubmed-meshheading:19812258-Parasite Egg Count, pubmed-meshheading:19812258-Recombinant Proteins, pubmed-meshheading:19812258-Schistosoma japonicum, pubmed-meshheading:19812258-Schistosomiasis japonica, pubmed-meshheading:19812258-Spleen, pubmed-meshheading:19812258-Vaccination, pubmed-meshheading:19812258-Vaccines, DNA
pubmed:year
2009
pubmed:articleTitle
DNA vaccination by electroporation and boosting with recombinant proteins enhances the efficacy of DNA vaccines for Schistosomiasis japonica.
pubmed:affiliation
Jiangsu Institute of Parasitic Diseases, Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Jiangsu Key Lab on Molecular Biology of Parasites, Wuxi 214064, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural