Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2009-10-6
pubmed:abstractText
Recent molecular-dynamics simulations have suggested that the arginine-rich HIV Tat peptides translocate by destabilizing and inducing transient pores in phospholipid bilayers. In this pathway for peptide translocation, Arg residues play a fundamental role not only in the binding of the peptide to the surface of the membrane, but also in the destabilization and nucleation of transient pores across the bilayer. Here we present a molecular-dynamics simulation of a peptide composed of nine Args (Arg-9) that shows that this peptide follows the same translocation pathway previously found for the Tat peptide. We test experimentally the hypothesis that transient pores open by measuring ionic currents across phospholipid bilayers and cell membranes through the pores induced by Arg-9 peptides. We find that Arg-9 peptides, in the presence of an electrostatic potential gradient, induce ionic currents across planar phospholipid bilayers, as well as in cultured osteosarcoma cells and human smooth muscle cells. Our results suggest that the mechanism of action of Arg-9 peptides involves the creation of transient pores in lipid bilayers and cell membranes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1542-0086
pubmed:author
pubmed:issnType
Electronic
pubmed:day
7
pubmed:volume
97
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1917-25
pubmed:dateRevised
2010-10-8
pubmed:meshHeading
pubmed-meshheading:19804722-Animals, pubmed-meshheading:19804722-Arginine, pubmed-meshheading:19804722-Cell Membrane, pubmed-meshheading:19804722-Cell Membrane Permeability, pubmed-meshheading:19804722-Cell Survival, pubmed-meshheading:19804722-Electric Conductivity, pubmed-meshheading:19804722-Gene Products, tat, pubmed-meshheading:19804722-Human Immunodeficiency Virus Proteins, pubmed-meshheading:19804722-Humans, pubmed-meshheading:19804722-Hydrogen-Ion Concentration, pubmed-meshheading:19804722-Molecular Conformation, pubmed-meshheading:19804722-Molecular Dynamics Simulation, pubmed-meshheading:19804722-Peptides, pubmed-meshheading:19804722-Phosphatidylcholines, pubmed-meshheading:19804722-Phosphatidylglycerols, pubmed-meshheading:19804722-Porosity, pubmed-meshheading:19804722-Protein Transport, pubmed-meshheading:19804722-Salts, pubmed-meshheading:19804722-Water
pubmed:year
2009
pubmed:articleTitle
Arginine-rich peptides destabilize the plasma membrane, consistent with a pore formation translocation mechanism of cell-penetrating peptides.
pubmed:affiliation
Department of Physics, Rensselaer Polytechnic Institute, Troy, New York, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural