Source:http://linkedlifedata.com/resource/pubmed/id/19800113
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2009-11-10
|
| pubmed:abstractText |
Systemic gene delivery systems are needed for therapeutic application to organs that are inaccessible by percutaneous injection. Currently, the main objective is the development of a stable and non-toxic vector that can encapsulate and deliver foreign genetic material to target cells. To this end, DNA, complexed with cationic lipids i.e. DOTAP/DOPE, was encapsulated into lipid nanocapsules (LNCs) leading to the formation of stable nanocarriers (DNA LNCs) with a size inferior to 130 nm. Amphiphilic and flexible poly (ethylene glycol) (PEG) polymer coatings [PEG lipid derivative (DSPE-mPEG(2000)) or F108 poloxamer] at different concentrations were selected to make DNA LNCs stealthy. Some of these coated lipid nanocapsules were able to inhibit complement activation and were not phagocytized in vitro by macrophagic THP-1 cells whereas uncoated DNA LNCs accumulated in the vacuolar compartment of THP-1 cells. These results correlated with a significant increase of in vivo circulation time in mice especially for DSPE-mPEG(2000) 10 mm and an early half-life time (t(1/2) of distribution) 5-fold greater than for non-coated DNA LNCs (7.1 h vs 1.4 h). Finally, a tumor accumulation assessed by in vivo fluorescence imaging system was evidenced for these coated LNCs as a passive targeting without causing any hepatic damage.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,2-distearoylphosphatidylethanolami...,
http://linkedlifedata.com/resource/pubmed/chemical/Complement System Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Lipids,
http://linkedlifedata.com/resource/pubmed/chemical/Nanocapsules,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylethanolamines
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
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| pubmed:issn |
1878-5905
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
31
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
321-9
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| pubmed:meshHeading |
pubmed-meshheading:19800113-Animals,
pubmed-meshheading:19800113-Cell Death,
pubmed-meshheading:19800113-Cell Line,
pubmed-meshheading:19800113-Complement System Proteins,
pubmed-meshheading:19800113-DNA,
pubmed-meshheading:19800113-Gene Transfer Techniques,
pubmed-meshheading:19800113-Genetic Vectors,
pubmed-meshheading:19800113-Humans,
pubmed-meshheading:19800113-Injections, Intravenous,
pubmed-meshheading:19800113-Kinetics,
pubmed-meshheading:19800113-Lipids,
pubmed-meshheading:19800113-Macrophages,
pubmed-meshheading:19800113-Mice,
pubmed-meshheading:19800113-Mice, Nude,
pubmed-meshheading:19800113-Microscopy, Fluorescence,
pubmed-meshheading:19800113-Nanocapsules,
pubmed-meshheading:19800113-Neoplasms,
pubmed-meshheading:19800113-Particle Size,
pubmed-meshheading:19800113-Phosphatidylethanolamines,
pubmed-meshheading:19800113-Surface Properties,
pubmed-meshheading:19800113-Time Factors,
pubmed-meshheading:19800113-Tissue Distribution
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| pubmed:year |
2010
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| pubmed:articleTitle |
Long-circulating DNA lipid nanocapsules as new vector for passive tumor targeting.
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| pubmed:affiliation |
Inserm U646, Université d'Angers, 10 rue André Boquel, F-49100 Angers, France.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|