| pubmed-article:19798124 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19798124 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:19798124 | lifeskim:mentions | umls-concept:C0040300 | lld:lifeskim |
| pubmed-article:19798124 | lifeskim:mentions | umls-concept:C0376358 | lld:lifeskim |
| pubmed-article:19798124 | lifeskim:mentions | umls-concept:C1706515 | lld:lifeskim |
| pubmed-article:19798124 | lifeskim:mentions | umls-concept:C1326205 | lld:lifeskim |
| pubmed-article:19798124 | lifeskim:mentions | umls-concept:C0334227 | lld:lifeskim |
| pubmed-article:19798124 | lifeskim:mentions | umls-concept:C1514559 | lld:lifeskim |
| pubmed-article:19798124 | lifeskim:mentions | umls-concept:C1704640 | lld:lifeskim |
| pubmed-article:19798124 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
| pubmed-article:19798124 | lifeskim:mentions | umls-concept:C0598934 | lld:lifeskim |
| pubmed-article:19798124 | lifeskim:mentions | umls-concept:C1999216 | lld:lifeskim |
| pubmed-article:19798124 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:19798124 | lifeskim:mentions | umls-concept:C0331858 | lld:lifeskim |
| pubmed-article:19798124 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:19798124 | pubmed:dateCreated | 2010-2-12 | lld:pubmed |
| pubmed-article:19798124 | pubmed:abstractText | The destruction of extracellular matrix by matrix metalloproteinases is a key event in cancer progression. The tissue inhibitors of metalloproteinases can restrain tumor growth by inhibiting these enzymes. We sought to determine whether overexpression of tissue inhibitor of metalloproteinase-3 (TIMP-3) could suppress the malignant phenotype of human prostate cancer cell line PC-3M. Stable overexpression of TIMP-3 inhibited cell proliferation significantly by MTT assay. Both early and late apoptosis were observed in TIMP-3 overexpressing cells, and flow cytometry analysis showed S-phase blocking of the cell cycle. Monolayer invasion assay and transwell invasion assay showed significantly decreased invasive potential in TIMP-3 overexpressing cells compared with control cells. Cell adhesion and motility were also lower after TIMP-3 was overexpressed. In vivo, cells stably overexpressing TIMP-3 completely lost the ability to form tumors after injection into nude mice. Transfection of TIMP-3 into established tumors by electroporation also had a significant antitumor effect. TIMP-3-treated tumor tissues had significant apoptosis by TUNEL assay. These results showed that overexpression of TIMP-3 inhibits invasion and proliferation of prostate cancer cells in vitro and inhibits tumor growth in vivo. The experiments suggest a potential use for TIMP-3 in the gene therapy of prostate cancer. | lld:pubmed |
| pubmed-article:19798124 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19798124 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19798124 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19798124 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19798124 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19798124 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:19798124 | pubmed:issn | 1476-5500 | lld:pubmed |
| pubmed-article:19798124 | pubmed:author | pubmed-author:LUMM | lld:pubmed |
| pubmed-article:19798124 | pubmed:author | pubmed-author:YUH WHW | lld:pubmed |
| pubmed-article:19798124 | pubmed:author | pubmed-author:LiuNN | lld:pubmed |
| pubmed-article:19798124 | pubmed:author | pubmed-author:ZhaoX JXJ | lld:pubmed |
| pubmed-article:19798124 | pubmed:author | pubmed-author:LiangZZ | lld:pubmed |
| pubmed-article:19798124 | pubmed:author | pubmed-author:ZhangLL | lld:pubmed |
| pubmed-article:19798124 | pubmed:author | pubmed-author:ZhanCC | lld:pubmed |
| pubmed-article:19798124 | pubmed:author | pubmed-author:ZhaoLL | lld:pubmed |
| pubmed-article:19798124 | pubmed:author | pubmed-author:FangXX | lld:pubmed |
| pubmed-article:19798124 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19798124 | pubmed:volume | 17 | lld:pubmed |
| pubmed-article:19798124 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19798124 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19798124 | pubmed:pagination | 171-9 | lld:pubmed |
| pubmed-article:19798124 | pubmed:meshHeading | pubmed-meshheading:19798124... | lld:pubmed |
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| pubmed-article:19798124 | pubmed:meshHeading | pubmed-meshheading:19798124... | lld:pubmed |
| pubmed-article:19798124 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:19798124 | pubmed:articleTitle | Inhibition of tumor growth and induction of apoptosis in prostate cancer cell lines by overexpression of tissue inhibitor of matrix metalloproteinase-3. | lld:pubmed |
| pubmed-article:19798124 | pubmed:affiliation | Department of Pathophysiology, Prostate Diseases Prevention and Treatment Research Centre, Norman Bethune Medical School, Jilin University, Changchun, China. | lld:pubmed |
| pubmed-article:19798124 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19798124 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
