Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3-4
|
pubmed:dateCreated |
1991-1-31
|
pubmed:abstractText |
A synthetic deoxyribonucleotide probe for virulent Listeria monocytogenes, designated ADO7, was evaluated for its ability to identify restriction fragments of L. monocytogenes with nucleic acid sequences homologous with the beta-hemolysin gene by Southern blot hybridization of clinical and food isolates. The synthetic probe hybridized with three restriction fragments (approximately 1.1, 0.86, and 0.76 kb) of the serotype 1/2A isolates. Southern blot hybridization of the serogroup 4B isolates indicated that the nucleic acid sequences homologous with the beta-hemolysin gene probe were limited to a single restriction fragment of approximately 1 kb.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Sep
|
pubmed:issn |
0378-1135
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
24
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
341-53
|
pubmed:dateRevised |
2010-11-18
|
pubmed:meshHeading |
pubmed-meshheading:1979702-Animals,
pubmed-meshheading:1979702-Blotting, Southern,
pubmed-meshheading:1979702-DNA, Bacterial,
pubmed-meshheading:1979702-DNA Probes,
pubmed-meshheading:1979702-Disease Outbreaks,
pubmed-meshheading:1979702-Food Microbiology,
pubmed-meshheading:1979702-Hemolysin Proteins,
pubmed-meshheading:1979702-Humans,
pubmed-meshheading:1979702-Listeria monocytogenes,
pubmed-meshheading:1979702-Listeriosis,
pubmed-meshheading:1979702-Nucleic Acid Hybridization,
pubmed-meshheading:1979702-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:1979702-Restriction Mapping,
pubmed-meshheading:1979702-Sequence Homology, Nucleic Acid
|
pubmed:year |
1990
|
pubmed:articleTitle |
Characterization of Listeria monocytogenes isolates by Southern blot hybridization.
|
pubmed:affiliation |
Physiopathology Research Unit, USDA-Agricultural Research Service, Ames, IA.
|
pubmed:publicationType |
Journal Article
|