Linked Life Data

19787410

Source:http://linkedlifedata.com/resource/pubmed/id/19787410

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-1-28
pubmed:abstractText
Transforming growth factor-betas (TGF-betas), expressed in various tissues, play important roles in embryonic development and adult tissue homeostasis through their effects on cell proliferation, cell differentiation, cell death, and cell motility. However, expression of TGF-beta signaling components and their biological effect on taste epithelia has not been elucidated. We performed expression analysis of TGF-beta signaling components in taste epithelia and found that the TGF-beta3 mRNA was specifically expressed in taste buds. Type II TGF-betas receptor (TbetaR-II) mRNA was specifically expressed in the tongue epithelia including the taste epithelia. To elucidate the biological function of TGF-beta3 in taste epithelia, we performed proliferation assay with primary cultured taste epithelial cells. In the presence of TGF-beta3, percentage of BrdU-labeled cells decreased significantly, suggesting that the TGF-beta3 inhibited the proliferation of cultured taste epithelial cells through inhibiting cell-cycle entry into S phase. By quantitative reverse transcription-polymerase chain reaction assay, we found that the TGF-beta3 resulted in an increased level of expression of p15Ink4b and p21Cip1, suggesting that the TGF-beta3 inhibited the taste epithelial cell proliferation through inhibiting G1cyclin-Cdk complexes. Taken together, these results suggested that the TGF-beta3 may regulate taste epithelial cell homeostasis through controlling cell proliferation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1543-706X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
36-44
pubmed:meshHeading
pubmed-meshheading:19787410-Animals, pubmed-meshheading:19787410-Cell Adhesion, pubmed-meshheading:19787410-Cell Proliferation, pubmed-meshheading:19787410-Cells, Cultured, pubmed-meshheading:19787410-Cyclin-Dependent Kinase Inhibitor p15, pubmed-meshheading:19787410-Cyclin-Dependent Kinase Inhibitor p21, pubmed-meshheading:19787410-Epithelial Cells, pubmed-meshheading:19787410-Gene Expression Regulation, pubmed-meshheading:19787410-Humans, pubmed-meshheading:19787410-Male, pubmed-meshheading:19787410-Mice, pubmed-meshheading:19787410-Mice, Inbred C57BL, pubmed-meshheading:19787410-Protein-Serine-Threonine Kinases, pubmed-meshheading:19787410-RNA, Messenger, pubmed-meshheading:19787410-Receptors, Transforming Growth Factor beta, pubmed-meshheading:19787410-Signal Transduction, pubmed-meshheading:19787410-Taste, pubmed-meshheading:19787410-Taste Buds, pubmed-meshheading:19787410-Transforming Growth Factor beta3
pubmed:year
2010
pubmed:articleTitle
TGF-beta3 is expressed in taste buds and inhibits proliferation of primary cultured taste epithelial cells.
pubmed:affiliation
Food Function Division, National Food Research Institute, National Agriculture and Food Research Organization, Kannondai 2-1-12, Tsukuba, Ibaraki 305-8642, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't