Linked Life Data

19787238

Source:http://linkedlifedata.com/resource/pubmed/id/19787238

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2009-9-29
pubmed:abstractText
A number of methods have been established for identifying sentinel nodes (SNs). In the present study, we attempted to clarify the immunological status of SNs with or without micrometastasis in breast cancer patients. SNs were identified by the dye- and gamma probe-guided method. Total RNA was extracted from the SNs, and the expression of T-BET, GATA-3, and FOXP3 were evaluated using quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Micrometastasis was identified as microscopically negative but positive by RT-PCR specific for mammaglobin. Of 88 patients, 17 (19.3%) showed positive metastasis in SNs (pN1, 14; pN2, 3). Of the 71 metastasis-negative SNs, 11 showed positive bands on RT-PCR specific for mamma-globin [pN0(mol+)]. There was no significant correlation among clinicopathological features with or without micrometastasis. Immunological parameters were compared among the 60 pN0, 11 pN0(mol+), and 17 pN1-2. Although T-BET expression was higher in pN0(mol+) than pN0, FOXP3 expression was also higher in pN0(mol+) than pN0. In pN1-2, T-BET expression decreased compared with pN0(mol+), but FOXP3 expression did not. On the other hand, GATA-3 expression inversely increased in pN1-2 compared with pN0(mol+). In patients with breast cancer, micrometastasis can stimulate Th1 response in SNs. However, the Treg cell response is also induced at the micrometastasis level and persists during the progression of metastasis in SNs. Then, the shift in the Th1/Th2 balance may preferentially lean toward Th2 responses in pN1-2 SNs and suppress antitumor immune responses. Micrometastasis [pN0(mol+)] is a status immunologically distinguishable from pN0 and pN1-2.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1021-335X
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1181-7
pubmed:meshHeading
pubmed-meshheading:19787238-Adenocarcinoma, pubmed-meshheading:19787238-Adult, pubmed-meshheading:19787238-Aged, pubmed-meshheading:19787238-Breast Neoplasms, pubmed-meshheading:19787238-Carcinoma, Intraductal, Noninfiltrating, pubmed-meshheading:19787238-Carcinoma, Papillary, pubmed-meshheading:19787238-Female, pubmed-meshheading:19787238-Forkhead Transcription Factors, pubmed-meshheading:19787238-GATA3 Transcription Factor, pubmed-meshheading:19787238-Gene Expression Regulation, Neoplastic, pubmed-meshheading:19787238-Humans, pubmed-meshheading:19787238-Immunoenzyme Techniques, pubmed-meshheading:19787238-Lymph Nodes, pubmed-meshheading:19787238-Lymphatic Metastasis, pubmed-meshheading:19787238-Middle Aged, pubmed-meshheading:19787238-Neoplasm Staging, pubmed-meshheading:19787238-Prognosis, pubmed-meshheading:19787238-RNA, Messenger, pubmed-meshheading:19787238-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:19787238-Sentinel Lymph Node Biopsy, pubmed-meshheading:19787238-T-Box Domain Proteins, pubmed-meshheading:19787238-T-Lymphocytes, Regulatory, pubmed-meshheading:19787238-Th1 Cells, pubmed-meshheading:19787238-Th2 Cells, pubmed-meshheading:19787238-Tumor Markers, Biological
pubmed:year
2009
pubmed:articleTitle
FOXP3 expression of micrometastasis-positive sentinel nodes in breast cancer patients.
pubmed:affiliation
Department of Surgical Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan. m79kazuo@saitama-med.ac.jp
pubmed:publicationType
Journal Article