Linked Life Data

19784593

Source:http://linkedlifedata.com/resource/pubmed/id/19784593

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2009-9-28
pubmed:abstractText
Lysophosphatidylcholine (LPC) is a bioactive lipid implicated to play a functional role in various diseases including atherosclerosis, diabetes, cancer, and inflammation. Conventional methods are of limited value for a systematic evaluation of LPC species concentrations due to complicated, time-consuming procedures. Here we describe a methodology based on electrospray ionization tandem mass spectrometry (ESI-MS/MS) applicable for high-throughput LPC species quantification. This assay provides accuracy and precision sufficient for the analysis of large clinical studies as well as basic biochemical studies in a broad range of biological material including plasma, tissues, and cell culture material. This method may be combined with methods based on the same analytical setup for glycerophospholipid, sphingolipid, and sterol analysis to evaluate LPC species in relation to other lipid species profiles.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1940-6029
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
580
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
29-37
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Quantification of lysophosphatidylcholine species by high-throughput electrospray ionization tandem mass spectrometry (ESI-MS/MS).
pubmed:affiliation
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't