Linked Life Data

19783639

Source:http://linkedlifedata.com/resource/pubmed/id/19783639

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-12-18
pubmed:abstractText
Gel-forming mucins are the largest complex glycoprotein macromolecules in the body. They form the matrix of gels protecting all the surface epithelia and are secreted as disulfide-bonded polymeric structures. The mechanisms by which they are formed and organized within cells and thereafter released to form mucus gels are not understood. In particular, the initial rate of expansion of the mucins after release from their secretory granules is very rapid (seconds), but no clear mechanism for how it is achieved has emerged. Our major interest is in lung mucins, but most particularly in MUC5B, which is the major gel-forming mucin in mucus, and which provides its major protective matrix. In this study, using OptiPrep density gradient ultracentrifugation, we have isolated a small amount of a stable form of the recently secreted and expanding MUC5B mucin, which accounts for less than 2% of the total mucin present. It has an average mass of approximately 150 x 10(6) Da and size Rg of 150 nm in radius of gyration. In transmission electron microscopy, this compact mucin has maintained a circular structure that is characterized by flexible chains connected around protein-rich nodes as determined by their ability to bind colloidal gold. The appearance indicates that the assembled mucins in a single granular form are organized around a number of nodes, each attached to four to eight subunits. The organization of the mucins in this manner is consistent with efficient packing of a number of large heavily glycosylated monomers while still permitting their rapid unfolding and hydration. For the first time, this provides some insight into how the carbohydrate regions might be organized around the NH(2)- and COOH-terminal globular protein domains within the granule and also explains how the mucin can expand so rapidly upon its release.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19783639-10820752, http://linkedlifedata.com/resource/pubmed/commentcorrection/19783639-10835316, http://linkedlifedata.com/resource/pubmed/commentcorrection/19783639-11537900, http://linkedlifedata.com/resource/pubmed/commentcorrection/19783639-1403682, http://linkedlifedata.com/resource/pubmed/commentcorrection/19783639-14228505, http://linkedlifedata.com/resource/pubmed/commentcorrection/19783639-17850209, http://linkedlifedata.com/resource/pubmed/commentcorrection/19783639-17850213, http://linkedlifedata.com/resource/pubmed/commentcorrection/19783639-17988208, http://linkedlifedata.com/resource/pubmed/commentcorrection/19783639-18182488, http://linkedlifedata.com/resource/pubmed/commentcorrection/19783639-1868152, http://linkedlifedata.com/resource/pubmed/commentcorrection/19783639-2184755, http://linkedlifedata.com/resource/pubmed/commentcorrection/19783639-2302163, http://linkedlifedata.com/resource/pubmed/commentcorrection/19783639-3476567, http://linkedlifedata.com/resource/pubmed/commentcorrection/19783639-3502764, http://linkedlifedata.com/resource/pubmed/commentcorrection/19783639-7195985, http://linkedlifedata.com/resource/pubmed/commentcorrection/19783639-78683, http://linkedlifedata.com/resource/pubmed/commentcorrection/19783639-9759493
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1522-1504
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
298
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
L15-22
pubmed:dateRevised
2011-7-19
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Unpacking a gel-forming mucin: a view of MUC5B organization after granular release.
pubmed:affiliation
1Department of Biochemistry and Biophysics and Cystic Fibrosis/Pulmonary Research and Treatment Center, Chapel Hill, NC, USA. kesimer@med.unc.edu
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural