Linked Life Data

1978305

Source:http://linkedlifedata.com/resource/pubmed/id/1978305

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1990-12-5
pubmed:abstractText
Eight 15I5 B-congenic White Leghorn chicken lines, containing haplotypes B2, B5, B12, B13, B15, B19, and B21, were subjected to molecular genotyping with chicken B-F (Class I) and B-L (Class II) major histocompatibility complex (MHC) probes. Genomic DNA was digested with restriction enzymes, hybridized with a Class I or Class II probe, and analyzed for restriction fragment length polymorphisms. Digestion with HindIII or EcoRI yielded no B-L polymorphisms. Digestion with PvuII or BglII and hybridization with a B-L or B-F probe produced polymorphisms that were shared between several haplotypes, although the haplotypes with similar patterns were clustered differently between Class I and Class II probes. The genetic variation seen for B-L and B-F probe hybridization of PvuII digests was much less than that previously demonstrated for B-G probing of PvuII digests of the same lines. Description of MHC Class I and II restriction patterns of the well-characterized 15I5 B-congenic lines will aid in identification of genes important in disease resistance.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0032-5791
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1195-203
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Analysis of restriction fragment length polymorphisms of the major histocompatibility complex of 15I5-B-congenic chicken lines.
pubmed:affiliation
Department of Animal Science, Iowa State University, Ames 50011.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't