Linked Life Data

19781051

Source:http://linkedlifedata.com/resource/pubmed/id/19781051

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-1-18
pubmed:abstractText
Grapevine (Vitis vinifera), the genome sequence of which has recently been reported, is considered as a model species to study fleshy fruit development and acid fruit physiology. Grape berry acidity is quantitatively and qualitatively affected upon increased K(+) accumulation, resulting in deleterious effects on fruit (and wine) quality. Aiming at identifying molecular determinants of K(+) transport in grapevine, we have identified a K(+) channel, named VvK1.1, from the Shaker family. In silico analyses indicated that VvK1.1 is the grapevine counterpart of the Arabidopsis AKT1 channel, known to dominate the plasma membrane inward conductance to K(+) in root periphery cells, and to play a major role in K(+) uptake from the soil solution. VvK1.1 shares common functional properties with AKT1, such as inward rectification (resulting from voltage sensitivity) or regulation by calcineurin B-like (CBL)-interacting protein kinase (CIPK) and Ca(2+)-sensing CBL partners (shown upon heterologous expression in Xenopus oocytes). It also displays distinctive features such as activation at much more negative membrane voltages or expression strongly sensitive to drought stress and ABA (upregulation in aerial parts, downregulation in roots). In roots, VvK1.1 is mainly expressed in cortical cells, like AKT1. In aerial parts, VvK1.1 transcripts were detected in most organs, with expression levels being the highest in the berries. VvK1.1 expression in the berry is localized in the phloem vasculature and pip teguments, and displays strong upregulation upon drought stress, by about 10-fold.VvK1.1 could thus play a major role in K(+) loading into berry tissues, especially upon drought stress.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1365-313X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
61
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
58-69
pubmed:meshHeading
pubmed-meshheading:19781051-Abscisic Acid, pubmed-meshheading:19781051-Arabidopsis Proteins, pubmed-meshheading:19781051-Computational Biology, pubmed-meshheading:19781051-Droughts, pubmed-meshheading:19781051-Fruit, pubmed-meshheading:19781051-Gene Expression Regulation, Plant, pubmed-meshheading:19781051-Genome, Plant, pubmed-meshheading:19781051-In Situ Hybridization, pubmed-meshheading:19781051-Phylogeny, pubmed-meshheading:19781051-Plant Components, Aerial, pubmed-meshheading:19781051-Plant Proteins, pubmed-meshheading:19781051-Plant Roots, pubmed-meshheading:19781051-Polymerase Chain Reaction, pubmed-meshheading:19781051-Potassium Channels, pubmed-meshheading:19781051-Protein-Serine-Threonine Kinases, pubmed-meshheading:19781051-Shaker Superfamily of Potassium Channels, pubmed-meshheading:19781051-Sodium Chloride, pubmed-meshheading:19781051-Vitis
pubmed:year
2010
pubmed:articleTitle
A grapevine Shaker inward K(+) channel activated by the calcineurin B-like calcium sensor 1-protein kinase CIPK23 network is expressed in grape berries under drought stress conditions.
pubmed:affiliation
UMR1083, Sciences pour l'OEnologie, INRA, 2 Place Viala, 34060 Montpellier Cedex 1, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't