Neural differentiation of embryonic stem (ES) cells is usually achieved by induction of ectoderm in embryoid bodies followed by the enrichment of neuronal progenitors using a variety of factors. Obtaining reproducible percentages of neural cells is difficult and the methods are time consuming.
Department of Otolarygology, EENT Hospital of Shanghai Medical College of Fudan University, Institute of Biomedical Sciences, Fudan University Shanghai, 200031, PR China. hwli@shmu.edu.cn