Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8A
pubmed:dateCreated
2009-9-25
pubmed:abstractText
Mitogenic regulation by caveolin-2 in response to insulin was investigated. Insulin triggered phosphorylation of caveolin-2 on tyrosine 19. Insulin increased the interaction between pY19-caveolin-2 and phospho-ERK, and that interaction was inhibited by a MEK inhibitor U0126. Insulin-induced interaction of caveolin-2 with phospho-ERK was prevented when tyrosine 19 is mutated to alanine. Insulin relocalized phospho-ERK and pY19-caveolin-2 to the nucleus and their nuclear co-localization was impaired by U0126. Down-regulation of caveolin-2 by caveolin-2 siRNA arrested the insulin-induced nuclear localization of ERK with no change in the insulin-stimulated ERK activation. Of consequence, the caveolin-2 siRNA attenuated the ERK-mediated c-Jun and cyclinD1 expression and DNA synthesis by insulin. In addition, actin cytoskeleton influenced the nuclear translocation of caveolin-2-ERK complex. Collectively, our findings underscore the importance of pY19-caveolin-2 with the spatial coordination by insulin in ERK-mediated mitogenic regulation of insulin signalling and indicate that the phosphorylation of pY19-caveolin-2 is required for actin cytoskeleton-dependent ERK nuclear import.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Actins, http://linkedlifedata.com/resource/pubmed/chemical/Caveolin 2, http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP..., http://linkedlifedata.com/resource/pubmed/chemical/Glucosides, http://linkedlifedata.com/resource/pubmed/chemical/Insulin, http://linkedlifedata.com/resource/pubmed/chemical/Nonidet P-40, http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine, http://linkedlifedata.com/resource/pubmed/chemical/Polyethylene Glycols, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-jun, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering, http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Insulin, http://linkedlifedata.com/resource/pubmed/chemical/octyl-beta-D-glucoside
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1582-4934
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1549-64
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:19778377-Actins, pubmed-meshheading:19778377-Animals, pubmed-meshheading:19778377-Caveolin 2, pubmed-meshheading:19778377-Cell Cycle, pubmed-meshheading:19778377-Cell Nucleus, pubmed-meshheading:19778377-Cell Proliferation, pubmed-meshheading:19778377-Cytoskeleton, pubmed-meshheading:19778377-Enzyme Activation, pubmed-meshheading:19778377-Extracellular Signal-Regulated MAP Kinases, pubmed-meshheading:19778377-Glucosides, pubmed-meshheading:19778377-Humans, pubmed-meshheading:19778377-Insulin, pubmed-meshheading:19778377-Mice, pubmed-meshheading:19778377-Phosphorylation, pubmed-meshheading:19778377-Phosphotyrosine, pubmed-meshheading:19778377-Polyethylene Glycols, pubmed-meshheading:19778377-Protein Binding, pubmed-meshheading:19778377-Protein Transport, pubmed-meshheading:19778377-Proto-Oncogene Proteins c-jun, pubmed-meshheading:19778377-RNA, Small Interfering, pubmed-meshheading:19778377-Rats, pubmed-meshheading:19778377-Receptor, Insulin, pubmed-meshheading:19778377-Solubility, pubmed-meshheading:19778377-Structure-Activity Relationship
pubmed:year
2009
pubmed:articleTitle
Identification of pY19-caveolin-2 as a positive regulator of insulin-stimulated actin cytoskeleton-dependent mitogenesis.
pubmed:affiliation
Department of Biochemistry, Division of Applied Life Science (BK21), Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't