rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8A
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pubmed:dateCreated |
2009-9-25
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pubmed:abstractText |
Mitogenic regulation by caveolin-2 in response to insulin was investigated. Insulin triggered phosphorylation of caveolin-2 on tyrosine 19. Insulin increased the interaction between pY19-caveolin-2 and phospho-ERK, and that interaction was inhibited by a MEK inhibitor U0126. Insulin-induced interaction of caveolin-2 with phospho-ERK was prevented when tyrosine 19 is mutated to alanine. Insulin relocalized phospho-ERK and pY19-caveolin-2 to the nucleus and their nuclear co-localization was impaired by U0126. Down-regulation of caveolin-2 by caveolin-2 siRNA arrested the insulin-induced nuclear localization of ERK with no change in the insulin-stimulated ERK activation. Of consequence, the caveolin-2 siRNA attenuated the ERK-mediated c-Jun and cyclinD1 expression and DNA synthesis by insulin. In addition, actin cytoskeleton influenced the nuclear translocation of caveolin-2-ERK complex. Collectively, our findings underscore the importance of pY19-caveolin-2 with the spatial coordination by insulin in ERK-mediated mitogenic regulation of insulin signalling and indicate that the phosphorylation of pY19-caveolin-2 is required for actin cytoskeleton-dependent ERK nuclear import.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Caveolin 2,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP...,
http://linkedlifedata.com/resource/pubmed/chemical/Glucosides,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Nonidet P-40,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Polyethylene Glycols,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-jun,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/octyl-beta-D-glucoside
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1582-4934
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1549-64
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:19778377-Actins,
pubmed-meshheading:19778377-Animals,
pubmed-meshheading:19778377-Caveolin 2,
pubmed-meshheading:19778377-Cell Cycle,
pubmed-meshheading:19778377-Cell Nucleus,
pubmed-meshheading:19778377-Cell Proliferation,
pubmed-meshheading:19778377-Cytoskeleton,
pubmed-meshheading:19778377-Enzyme Activation,
pubmed-meshheading:19778377-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:19778377-Glucosides,
pubmed-meshheading:19778377-Humans,
pubmed-meshheading:19778377-Insulin,
pubmed-meshheading:19778377-Mice,
pubmed-meshheading:19778377-Phosphorylation,
pubmed-meshheading:19778377-Phosphotyrosine,
pubmed-meshheading:19778377-Polyethylene Glycols,
pubmed-meshheading:19778377-Protein Binding,
pubmed-meshheading:19778377-Protein Transport,
pubmed-meshheading:19778377-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:19778377-RNA, Small Interfering,
pubmed-meshheading:19778377-Rats,
pubmed-meshheading:19778377-Receptor, Insulin,
pubmed-meshheading:19778377-Solubility,
pubmed-meshheading:19778377-Structure-Activity Relationship
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pubmed:year |
2009
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pubmed:articleTitle |
Identification of pY19-caveolin-2 as a positive regulator of insulin-stimulated actin cytoskeleton-dependent mitogenesis.
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pubmed:affiliation |
Department of Biochemistry, Division of Applied Life Science (BK21), Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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