Linked Life Data

19777575

Source:http://linkedlifedata.com/resource/pubmed/id/19777575

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-4-14
pubmed:abstractText
Scaffolds exhibiting biological recognition and specificity play an important role in tissue engineering and regenerative medicine. The bioactivity of scaffolds in turn influences, directs, or manipulates cellular responses. In this study, chitosan/poly(lactic acid-co-glycolic acid) (chitosan/PLAGA) sintered microsphere scaffolds were functionalized via heparin immobilization. Heparin was successfully immobilized on chitosan/PLAGA scaffolds with controllable loading efficiency. Mechanical testing showed that heparinization of chitosan/PLAGA scaffolds did not significantly alter the mechanical properties and porous structures. In addition, the heparinized chitosan/PLAGA scaffolds possessed a compressive modulus of 403.98 +/- 19.53 MPa and a compressive strength of 9.83 +/- 0.94 MPa, which are in the range of human trabecular bone. Furthermore, the heparinized chitosan/PLAGA scaffolds had an interconnected porous structure with a total pore volume of 30.93 +/- 0.90% and a median pore size of 172.33 +/- 5.89 mum. The effect of immobilized heparin on osteoblast-like MC3T3-E1 cell growth was investigated. MC3T3-E1 cells proliferated three dimensionally throughout the porous structure of the scaffolds. Heparinized chitosan/PLAGA scaffolds with low heparin loading (1.7 microg/scaffold) were shown to be capable of stimulating MC3T3-E1 cell proliferation by MTS assay and cell differentiation as evidenced by elevated osteocalcin expression when compared with nonheparinized chitosan/PLAGA scaffold and chitosan/PLAGA scaffold with high heparin loading (14.1 microg/scaffold). This study demonstrated the potential of functionalizing chitosan/PLAGA scaffolds via heparinization with improved cell functions for bone tissue engineering applications.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1552-4965
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
93
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1193-208
pubmed:meshHeading
pubmed-meshheading:19777575-Adsorption, pubmed-meshheading:19777575-Alkaline Phosphatase, pubmed-meshheading:19777575-Animals, pubmed-meshheading:19777575-Bone and Bones, pubmed-meshheading:19777575-Cell Proliferation, pubmed-meshheading:19777575-Cell Survival, pubmed-meshheading:19777575-Chitosan, pubmed-meshheading:19777575-Compressive Strength, pubmed-meshheading:19777575-Heparin, pubmed-meshheading:19777575-Immobilized Proteins, pubmed-meshheading:19777575-Lactic Acid, pubmed-meshheading:19777575-Mice, pubmed-meshheading:19777575-Microscopy, Electron, Scanning, pubmed-meshheading:19777575-Microspheres, pubmed-meshheading:19777575-Osteoblasts, pubmed-meshheading:19777575-Osteocalcin, pubmed-meshheading:19777575-Photoelectron Spectroscopy, pubmed-meshheading:19777575-Polyglycolic Acid, pubmed-meshheading:19777575-Porosity, pubmed-meshheading:19777575-Solubility, pubmed-meshheading:19777575-Surface Properties, pubmed-meshheading:19777575-Tissue Engineering, pubmed-meshheading:19777575-Tissue Scaffolds
pubmed:year
2010
pubmed:articleTitle
Functionalization of chitosan/poly(lactic acid-glycolic acid) sintered microsphere scaffolds via surface heparinization for bone tissue engineering.
pubmed:affiliation
Department of Chemical Engineering, University of Virginia, Charlottesville, Virginia 22904, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.