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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1990-12-10
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pubmed:abstractText |
13 cases of childhood acute lymphoblastic leukaemia (ALL) were studied combining cell surface marker analysis with immunogenotyping by Southern blot hybridisation with a panel of antigen receptor gene probes. The immunophenotypes were unequivocal: 7 patients had B-phenotype and 6 patients T-phenotype ALL. In several patients immunogenotypes were not fully consistent with the respective phenotypes. For example, 2 B-cell precursor ALL had rearranged TCR beta chain genes and 2 T-ALL rearrangement of Ig heavy-chain genes. All cases showed clonal rearrangement or deletions within the TCR delta gene locus. TCR delta gene rearrangements might, therefore, serve as markers of clonality but not of B- or T-lineage in immature lymphoid neoplasms. We conclude that in current diagnostic practice immunogenotyping is a supplement rather than an alternative to immunophenotyping by surface marker analysis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/BglII endonuclease,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonuclease EcoRI,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonuclease HindIII,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonucleases, Type II...,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Heavy Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0902-4441
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
215-22
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:1977612-Adolescent,
pubmed-meshheading:1977612-Bacterial Proteins,
pubmed-meshheading:1977612-Burkitt Lymphoma,
pubmed-meshheading:1977612-Child,
pubmed-meshheading:1977612-Child, Preschool,
pubmed-meshheading:1977612-DNA Probes,
pubmed-meshheading:1977612-Deoxyribonuclease EcoRI,
pubmed-meshheading:1977612-Deoxyribonuclease HindIII,
pubmed-meshheading:1977612-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:1977612-Gene Rearrangement,
pubmed-meshheading:1977612-Gene Rearrangement, T-Lymphocyte,
pubmed-meshheading:1977612-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor,
pubmed-meshheading:1977612-Genotype,
pubmed-meshheading:1977612-Humans,
pubmed-meshheading:1977612-Immunoglobulin Heavy Chains,
pubmed-meshheading:1977612-Immunophenotyping,
pubmed-meshheading:1977612-Infant,
pubmed-meshheading:1977612-Leukemia-Lymphoma, Adult T-Cell,
pubmed-meshheading:1977612-Nucleic Acid Hybridization,
pubmed-meshheading:1977612-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:1977612-Receptors, Antigen, T-Cell
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pubmed:year |
1990
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pubmed:articleTitle |
Immunogenotyping with antigen receptor gene probes as a diagnostic tool in childhood acute lymphoblastic leukaemia.
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pubmed:affiliation |
Institute of Medical Oncology, Inselspital, Bern, Switzerland.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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