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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2010-2-1
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pubmed:abstractText |
Polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA) and arachidonic acid (AA), are the main components of the phospholipids, in cerebral membranes. A dietary-induced cerebral DHA deficit results in altered behaviour and neurotransmission in rodents. To determine whether PUFA were acting on the neurotransmitter release machinery, we measured the release of [(3)H]-noradrenaline (NA) from SH-SY5Y neuroblastoma cells with modified PUFA membrane contents and from cells incubated with medium containing high DHA or AA. The membranes of cells incubated with 70 microM DHA for 3 days had 7.6-times more DHA in their ethanolamine glycerophospholipids, while the membranes of cells incubated with AA had 40% less. Incorporation of DHA enhanced basal [(3)H]-NA release (25%, p<0.05), but not KCl-evoked [(3)H]-NA release. Brief incubation with DHA during vesicle mobilization also strongly increased [3H]-NA release. AA had no effect. The genes encoding for the calcium sensor synaptotagmin 1, and for the two SNARE complex proteins syntaxin 1A and synaptobrevin 1 were not affected by PUFA incorporation, as indicated by assays for specific mRNAs and proteins. Thus both a high membrane DHA content and free DHA in the medium enhance the release of [(3)H]-NA from SH-SY5Y cells. This suggests that brain membrane DHA influences exocytosis, which then regulates neurotransmission.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1872-9754
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pubmed:author |
pubmed-author:BénédicteLangelierL,
pubmed-author:DenisIsabelleI,
pubmed-author:DenisStéphanieS,
pubmed-author:GéraldineMathieuM,
pubmed-author:IsabelleDenisD,
pubmed-author:LangelierBénédicteB,
pubmed-author:LavialleMoniqueM,
pubmed-author:MathieuGéraldineG,
pubmed-author:MoniqueLavialleL,
pubmed-author:StéphanieDenisD,
pubmed-author:SylvieVancasselV,
pubmed-author:VancasselSylvieS
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pubmed:copyrightInfo |
2009 Elsevier Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
94-100
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pubmed:dateRevised |
2010-10-4
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pubmed:meshHeading |
pubmed-meshheading:19770016-Brain,
pubmed-meshheading:19770016-Brain Chemistry,
pubmed-meshheading:19770016-Cell Line, Tumor,
pubmed-meshheading:19770016-Cell Membrane,
pubmed-meshheading:19770016-Docosahexaenoic Acids,
pubmed-meshheading:19770016-Exocytosis,
pubmed-meshheading:19770016-Glycerophospholipids,
pubmed-meshheading:19770016-Humans,
pubmed-meshheading:19770016-Membrane Lipids,
pubmed-meshheading:19770016-Nerve Tissue Proteins,
pubmed-meshheading:19770016-Neurons,
pubmed-meshheading:19770016-Norepinephrine,
pubmed-meshheading:19770016-Presynaptic Terminals,
pubmed-meshheading:19770016-RNA, Messenger,
pubmed-meshheading:19770016-Synaptic Transmission,
pubmed-meshheading:19770016-Synaptic Vesicles,
pubmed-meshheading:19770016-Tritium,
pubmed-meshheading:19770016-Up-Regulation
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pubmed:year |
2010
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pubmed:articleTitle |
DHA enhances the noradrenaline release by SH-SY5Y cells.
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pubmed:affiliation |
INRA, Unité de Nutrition et Régulation Lipidiques des Fonctions Cérébrales (NuRéLiCe), UR909, Domaine de Vilvert, Jouy en Josas, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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