Linked Life Data

19763908

Source:http://linkedlifedata.com/resource/pubmed/id/19763908

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-1-21
pubmed:abstractText
We previously characterized a C2H2-type zinc finger protein HZF1 (ZNF16) and demonstrated its important role in erythroid and megakaryocytic differentiation. This protein was located in nucleus. In this study, we first approved that HZF1 solely could activate lacZ reporter gene in yeast host Y190. This self-activation phenomenon together with structure and distribution of HZF1 suggested it as a potential transcription factor. By the auto-activation experiments and the luciferase reporter system and deletion mutation analysis, we further located the trans-activation domain at amino acid residences 49-197 within the non-zinc finger region of HZF1. An acidic residue-rich subregion (amino acids 49-105) was important for the trans-activation effect, but it could not function independently. By deletion mutation analysis, we also identified three nuclear location signals, which were located in the regions of amino acids 255-280, 328-360, and 460-490, respectively, and all of them within the zinc finger region.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1559-0305
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
83-9
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Identification of the trans-activation domain and the nuclear location signals of human zinc finger protein HZF1 (ZNF16).
pubmed:affiliation
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Dong Dan San Tiao, Beijing, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't