Linked Life Data

19763779

Source:http://linkedlifedata.com/resource/pubmed/id/19763779

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2010-1-29
pubmed:databankReference
pubmed:abstractText
Caliciviruses causing diarrhea have been reported in both industrial and developing countries, including China, in recent years. Porcine caliciviruses that are closely related to human sapoviruses (SaVs) and noroviruses (NoVs) have also been detected in swine, which has raised discussion about the animal reservoir and the potential risk for zoonotic transmission to humans. The objective of this work was to determine the frequency and age distribution of SaVs and NoVs in pigs and to characterize the strains prevalent in eastern China. A total of 904 stool samples from pigs of different ages were collected from eastern China from April 2008 to March 2009 and tested for both SaVs and NoVs using reverse transcription-polymerase chain reaction (RT-PCR). Our results indicate that 8 (0.9%) stool samples were positive for SaVs and 2 (0.2%) for NoVs. Phylogenetic analysis of partial sequences of the RNA-dependent RNA polymerase (RdRp) gene indicated that all of the SaV strains belonged to the GIII SaVs, while the two NoV strains belonged to the GII NoV genogroup. The 8 SaV strains were further divided into two clusters, which clustered closely with the Netherlands isolate (AY615804) and the Chinese strain (EU599212), respectively. The two NoV strains shared about 67.3-67.6% nucleotide homology with a human norovirus strain (DQ369797), the only NoV strain from mainland China available in GenBank. Moreover, our results suggest that SaV infections are more frequent in 0-1 month-old pigs than in older ones. In conclusion, the present study provides evidence that PoSaVs and PoNoVs exist in swine in eastern China.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
1432-8798
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
154
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1625-30
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Molecular detection and prevalence of porcine caliciviruses in eastern China from 2008 to 2009.
pubmed:affiliation
Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai JiaoTong University, Shanghai, People's Republic of China. njnushenquan@yahoo.com.cn
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't