Source:http://linkedlifedata.com/resource/pubmed/id/19763421
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2009-9-18
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| pubmed:abstractText |
Bacillus thuringiensis, an entomopathogenic bacterium belonging to the B. cereus group, harbors numerous extra-chromosomal DNA molecules whose sizes range from 2 to 250 kb. In this study, we used a plasmid capture system (PCS) to clone three small plasmids from B. thuringiensis subsp. kurstaki Kl which were not found in B. thuringiensis subsp. kurstaki HD-1, and determined the complete nucleotide sequence of plasmid pKlS-1 (5.5 kb). Of the six putative open reading frames (ORF2-ORF7) in pKlS-1, ORF2 (MobKl) showed approximately 90% aa identity with the Mob-proteins of pGI2 and pTX14-2, which are rolling circle replicating group VII (RCR group VII) plasmids from B. thuringiensis. In addition, a putative origin of transfer (oriT) showed 95.8% identity with those of pGI2 and pTX14-2. ORF3 (RepK1) showed relatively low aa identity (17.8-25.2%) with the Rep protein coded by RCR plasmids, however. The putative double-strand origin of replication (dso) and single-strand origin of replication (sso) of pKlS-1 exhibited approximately 70% and 64% identities with those of pGI2 and pTX14-2. ORF6 and 7 showed greater than 50% similarities with alkaline serine protease, which belongs to the subtilase family. The other 2 ORFs were identified as hypothetical proteins. To determine the replicon of pKlS-1, seven subclones were contructed in the B. thuringiensis ori-negative pHTIK vector and were electroporated into a plasmid cured B. thuringiensis strain. The 1.6 kb region that included the putative ORF3 (ReplK), dso and ORF4, exhibited replication ability. These findings identified pKlS-1 as a new RCR group VII plasmid, and determined its replication region.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
1976-3794
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| pubmed:author |
pubmed-author:ChoiJae YoungJY,
pubmed-author:JeYeon HoYH,
pubmed-author:KimYang-SuYS,
pubmed-author:KuzT JTJ,
pubmed-author:LiMing ShunMS,
pubmed-author:LiuZi DuoZD,
pubmed-author:RohJong YulJY,
pubmed-author:ShimHee JinHJ,
pubmed-author:TaoXueyingX,
pubmed-author:WangYongY,
pubmed-author:XuHong GuangHG,
pubmed-author:YuZi NiuZN
|
| pubmed:issnType |
Electronic
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| pubmed:volume |
47
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
466-72
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| pubmed:meshHeading |
pubmed-meshheading:19763421-Bacillus thuringiensis,
pubmed-meshheading:19763421-Base Sequence,
pubmed-meshheading:19763421-Cloning, Molecular,
pubmed-meshheading:19763421-DNA Replication,
pubmed-meshheading:19763421-Molecular Sequence Data,
pubmed-meshheading:19763421-Open Reading Frames,
pubmed-meshheading:19763421-Plasmids,
pubmed-meshheading:19763421-Replication Origin,
pubmed-meshheading:19763421-Sequence Alignment
|
| pubmed:year |
2009
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| pubmed:articleTitle |
Cloning and molecular characterization of a novel rolling-circle replicating plasmid, pK1S-1, from Bacillus thuringiensis subsp. kurstaki K1.
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| pubmed:affiliation |
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, P. R. China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|