Linked Life Data

19763084

Source:http://linkedlifedata.com/resource/pubmed/id/19763084

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
2009-11-5
pubmed:abstractText
Selective protein export from the endoplasmic reticulum is mediated by COPII vesicles. Here, we investigated the dynamics of fluorescently labelled cargo and non-cargo proteins during COPII vesicle formation using single-molecule microscopy combined with an artificial planar lipid bilayer. Single-molecule analysis showed that the Sar1p-Sec23/24p-cargo complex, but not the Sar1p-Sec23/24p complex, undergoes partial dimerization before Sec13/31p recruitment. On addition of a complete COPII mixture, cargo molecules start to assemble into fluorescent spots and clusters followed by vesicle release from the planar membrane. We show that continuous GTPase cycles of Sar1p facilitate cargo concentration into COPII vesicle buds, and at the same time, non-cargo proteins are excluded from cargo clusters. We propose that the minimal set of COPII components is required not only to concentrate cargo molecules, but also to mediate exclusion of non-cargo proteins from the COPII vesicles.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1460-2075
pubmed:author
pubmed:issnType
Electronic
pubmed:day
4
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3279-89
pubmed:dateRevised
2010-11-5
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane.
pubmed:affiliation
Department of Biomolecular Energetics, The Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't