|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
18
|
|
pubmed:dateCreated |
1990-10-24
|
|
pubmed:abstractText |
Immunoprecipitation of Thy-1 from Triton X-100 detergent lysates of surface-iodinated and chemically cross-linked T cells precipitated at least five major and discrete bands. Four of these bands were identified as Thy-1, CD45 (a transmembrane tyrosine phosphatase), a major histocompatibility complex-encoded class I molecule, and beta 2-microglobulin. Similar analyses revealed that CD45 was coprecipitated from lysates of cross-linker-treated cells by antibodies to the T-cell antigen receptor (TCR). The same pattern of coprecipitated bands was observed when digitonin was used to lyse untreated cells. Immunoprecipitation of Thy-1 or the TCR from lysates of cross-linked T cells precipitated CD45 tyrosine phosphatase activity. Calculations based upon the amounts of coprecipitated enzymatic activity or TCR zeta chain indicate that a substantial fraction of Thy-1 and TCR complexes can be cross-linked to CD45. These data support a model in which the dependence of Thy-1 signaling on TCR coexpression is due to their common interaction with a tyrosine phosphatase and provide a possible structural basis for the influence of CD45 on TCR-mediated signaling.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-1108012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-1168492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-1980615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2188661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2428504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2467820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2523715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2525147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2530588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2548204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2550143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2786526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2845400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2845582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2853967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2865681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2895789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2950524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2952710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2960536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2962345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2967767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2973067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-2982803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-307764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-3160781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-3278811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-3491868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-47173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-6131095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-6142077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-6184304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-6603688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-6886418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-7194313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1976251-81133
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD45,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Thy-1,
http://linkedlifedata.com/resource/pubmed/chemical/Cross-Linking Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoprotein Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
87
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
7085-9
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:1976251-Animals,
pubmed-meshheading:1976251-Antibodies, Monoclonal,
pubmed-meshheading:1976251-Antigens, CD45,
pubmed-meshheading:1976251-Antigens, Differentiation,
pubmed-meshheading:1976251-Antigens, Surface,
pubmed-meshheading:1976251-Antigens, Thy-1,
pubmed-meshheading:1976251-Cell Membrane,
pubmed-meshheading:1976251-Cross-Linking Reagents,
pubmed-meshheading:1976251-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1976251-Histocompatibility Antigens,
pubmed-meshheading:1976251-Kinetics,
pubmed-meshheading:1976251-Membrane Glycoproteins,
pubmed-meshheading:1976251-Mice,
pubmed-meshheading:1976251-Mice, Inbred C3H,
pubmed-meshheading:1976251-Phosphoprotein Phosphatases,
pubmed-meshheading:1976251-Protein Binding,
pubmed-meshheading:1976251-Protein Tyrosine Phosphatases,
pubmed-meshheading:1976251-Receptors, Antigen, T-Cell,
pubmed-meshheading:1976251-Spleen,
pubmed-meshheading:1976251-T-Lymphocytes
|
|
pubmed:year |
1990
|
|
pubmed:articleTitle |
Intimate association of Thy-1 and the T-cell antigen receptor with the CD45 tyrosine phosphatase.
|
|
pubmed:affiliation |
Biological Response Modifiers Program, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
