Source:http://linkedlifedata.com/resource/pubmed/id/19762485
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
20
|
| pubmed:dateCreated |
2009-12-7
|
| pubmed:abstractText |
Marrow stromal cells (MSCs) provide important survival and drug resistance signals to chronic lymphocytic leukemia (CLL) cells, but current models to analyze CLL-MSC interactions are heterogeneous. Therefore, we tested different human and murine MSC lines and primary human MSCs for their ability to protect CLL cells from spontaneous and drug-induced apoptosis. Our results show that both human and murine MSCs are equally effective in protecting CLL cells from fludarabine-induced apoptosis. This protective effect was sustained over a wide range of CLL-MSC ratios (5:1 to 100:1), and the levels of protection were reproducible in 4 different laboratories. Human and murine MSCs also protected CLL cells from dexamethasone- and cyclophosphamide-induced apoptosis. This protection required cell-cell contact and was virtually absent when CLL cells were separated from the MSCs by micropore filters. Furthermore, MSCs maintained Mcl-1 and protected CLL cells from spontaneous and fludarabine-induced Mcl-1 and PARP cleavage. Collectively, these studies define common denominators for CLL cocultures with MSCs. They also provide a reliable, validated tool for future investigations into the mechanism of MSC-CLL cross talk and for drug testing in a more relevant fashion than the commonly used suspension cultures.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
1528-0020
|
| pubmed:author |
pubmed-author:BalakrishnanKumudhaK,
pubmed-author:BurgerJan AJA,
pubmed-author:ChenRongR,
pubmed-author:DingWeiW,
pubmed-author:EstrovZeevZ,
pubmed-author:GandhiVarshaV,
pubmed-author:JägerUlrichU,
pubmed-author:KayNeil ENE,
pubmed-author:KeatingMichael JMJ,
pubmed-author:KurtovaAntonina VAV,
pubmed-author:PlunkettWilliamW,
pubmed-author:QuirogaMaite PMP,
pubmed-author:SchnablSusanneS,
pubmed-author:ShehataMedhatM,
pubmed-author:SivinaMarielaM,
pubmed-author:WierdaWilliam GWG
|
| pubmed:issnType |
Electronic
|
| pubmed:day |
12
|
| pubmed:volume |
114
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4441-50
|
| pubmed:meshHeading |
pubmed-meshheading:19762485-Animals,
pubmed-meshheading:19762485-Antineoplastic Agents,
pubmed-meshheading:19762485-Apoptosis,
pubmed-meshheading:19762485-Blotting, Western,
pubmed-meshheading:19762485-Bone Marrow Cells,
pubmed-meshheading:19762485-Cell Adhesion,
pubmed-meshheading:19762485-Cell Communication,
pubmed-meshheading:19762485-Cell Line, Tumor,
pubmed-meshheading:19762485-Cells, Cultured,
pubmed-meshheading:19762485-Coculture Techniques,
pubmed-meshheading:19762485-Drug Resistance, Neoplasm,
pubmed-meshheading:19762485-Flow Cytometry,
pubmed-meshheading:19762485-Humans,
pubmed-meshheading:19762485-Leukemia, Lymphocytic, Chronic, B-Cell,
pubmed-meshheading:19762485-Mice,
pubmed-meshheading:19762485-Stromal Cells
|
| pubmed:year |
2009
|
| pubmed:articleTitle |
Diverse marrow stromal cells protect CLL cells from spontaneous and drug-induced apoptosis: development of a reliable and reproducible system to assess stromal cell adhesion-mediated drug resistance.
|
| pubmed:affiliation |
Department of Leukemia, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77230-1402, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|