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pubmed:abstractText |
Ileal mucus and epithelial cells were isolated from newborn piglets that had never been fed and 35-day-old unweaned piglets. Both newborn and 35-day-old piglet mucus preparations supported growth of Escherichia coli Bd 1107/75 08, a K88-fimbriated porcine enterotoxigenic strain, equally well (i.e., generation times of 28 min were observed in both cases). Adhesion of E. coli Bd 1107/75 08 to 35-day-old piglet ileal epithelial cells was, at most, 2 times that of the same strain to newborn piglet ileal epithelial cells; however, adhesion of E. coli Bd 1107/75 08 to 35-day-old piglet ileal mucus was 16 times that of the same strain to newborn piglet ileal mucus. The receptor in 35-day-old piglet ileal mucus was K88 specific, since it could be removed by purified K88ab fimbriae. Furthermore, adhesion of E. coli Bd 1107/75 08 to 35-day-old piglet ileal mucus was blocked by PAB10, a K88ab-, K88ac-, K88ad-specific monoclonal antibody. Although E. coli Bd 1107/75 08 traversed both newborn and 35-day-old piglet ileal mucus about equally well in vitro and bound well to underlying ileal epithelial cells after passing through newborn ileal mucus, it did not bind to ileal epithelial cells after passing through 35-day-old piglet ileal mucus. The data are discussed with respect to the role that K88-specific receptors present in newborn and ileal mucus might play in the pathogenesis of porcine enterotoxigenic E. coli strains which bear K88 fimbriae.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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