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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2010-1-29
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pubmed:abstractText |
Neuronal death induced by serum deprivation (SD) in HT22-cells was accompanied by a moderate activation of caspase-3, a prominent upregulation of AIF and its translocation into the nucleus. In addition protein levels of autophagy markers such as LC3 and beclin-1 were affected by SD. The ratio of LC3-II/LC3-I was significantly increased in serum deprived cultures. Furthermore, the addition of the pan-caspase inhibitor z-VAD(OMe)-FMK (zVAD) does not protect HT22-cells from SD-induced neurodegeneration. However, addition of the autophagy inhibitors such as 3-methyladenine (3-MA) or bafilomycin A1 (BafA1) provided a potentiation of cell death induced by SD. z-VAD and 3-MA in combination were not only ineffective in rescuing cells from the damaging effects of SD, but seem likely to act in synergy to potentiate slightly SD-induced cell death. The results of the current study suggest that SD induced predominantly caspase-independent apoptosis in hippocampal HT22 cells and that inhibition of autophagy is rather deleterious than protective.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3-methyladenine,
http://linkedlifedata.com/resource/pubmed/chemical/Adenine,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Chloromethyl Ketones,
http://linkedlifedata.com/resource/pubmed/chemical/Apoptosis Regulatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Becn1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/MAP1LC3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Macrolides,
http://linkedlifedata.com/resource/pubmed/chemical/Microtubule-Associated Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/bafilomycin A1,
http://linkedlifedata.com/resource/pubmed/chemical/z-Val-Ala-Asp(Ome)-fluoromethylketon...
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1573-675X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1274-88
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pubmed:meshHeading |
pubmed-meshheading:19757066-Adenine,
pubmed-meshheading:19757066-Amino Acid Chloromethyl Ketones,
pubmed-meshheading:19757066-Animals,
pubmed-meshheading:19757066-Apoptosis,
pubmed-meshheading:19757066-Apoptosis Regulatory Proteins,
pubmed-meshheading:19757066-Autophagy,
pubmed-meshheading:19757066-Caspase 3,
pubmed-meshheading:19757066-Cell Line,
pubmed-meshheading:19757066-Culture Media, Serum-Free,
pubmed-meshheading:19757066-Hippocampus,
pubmed-meshheading:19757066-Macrolides,
pubmed-meshheading:19757066-Mice,
pubmed-meshheading:19757066-Microtubule-Associated Proteins,
pubmed-meshheading:19757066-Nerve Degeneration
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pubmed:year |
2009
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pubmed:articleTitle |
Serum deprivation induced autophagy and predominantly an AIF-dependent apoptosis in hippocampal HT22 neurons.
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pubmed:affiliation |
Institute of Cellular and Molecular Anatomy, Clinics of the Wolfgang Goethe-University, Frankfurt/Main, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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