Linked Life Data

1975608

Source:http://linkedlifedata.com/resource/pubmed/id/1975608

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1990-10-11
pubmed:abstractText
We have recently demonstrated by dielectric relaxation studies in the radio-frequency range that there is a sharp decrease in the conductivity and permittivity of the membranes of chick embryo myoblasts in vitro at the time of fusion (60 h) (Bonincontro et al. 1987). This sharp fall in membrane electrical parameters was subsequently shown to be due to changes in ionic flux, particularly of the Na+/K+ equilibrium (Santini et al. 1988). Ionizing radiation induces a wide variety of effects on biological membranes, including variations in membrane ionic transport. We wished to investigate if sublethal doses of gamma-irradiation could affect membrane electrical parameters and thus myoblast membrane fusion. Consequently, chick embryo myoblast aggregate cultures were irradiated with 3.25, 5.15 or 6.35 Gy at 24 h of culture. We found that the lower dose delays membrane fusion by about 10 h while the two higher doses block fusion up to 120 h of culture. Aggregates showed a very high cell viability. The possible mechanisms by which ionizing radiation causes these variations in myoblast membrane electrical properties and fusion are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0955-3002
pubmed:author
pubmed:issnType
Print
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
463-74
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Dose-dependent effects of ionizing radiation on in vitro myoblast fusion.
pubmed:affiliation
Laboratorio di Biologia Cellulare, Istituto Superiore di Sanità, Rome, Italy.
pubmed:publicationType
Journal Article, In Vitro