Linked Life Data

19755115

Source:http://linkedlifedata.com/resource/pubmed/id/19755115

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-10-14
pubmed:abstractText
TK0306 (Tk-DeaD) of hyperthermophilic archaeon Thermococcus kodakaraensis is annotated as the DEAD-box helicase gene; nevertheless, its ortholog has not been identified in closely related genera, Pyrococcus spp., which generally grow at higher temperature than T. kodakaraensis, suggesting that the cold-inducible RNA helicase of Tk-DeaD functions under cold stress conditions. Quantitative RT-PCR revealed that Tk-deaD was more dominantly transcribed at 60 degrees C than at 85 degrees C and 93 degrees C in both logarithmic and stationary phases. Immunoblot analyses revealed that Tk-DeaD was detected only in logarithmic-phase cells cultivated at 60 degrees C but hardly detected at 85 degrees C and 93 degrees C in both phases. Tk-DeaD expression is, hence, post-transcriptionally regulated and appears under vigorous growth conditions at 60 degrees C. Recombinant Tk-DeaD purified to homogeneity started to unfold at 20 degrees C, fully unfolded at 70 degrees C, and exhibited maximal ATPase activity and unwinding activity specific for single-strand paired RNA at 50 degrees C, which is lower than the growth limit of T. kodakaraensis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1090-2104
pubmed:author
pubmed:issnType
Electronic
pubmed:day
27
pubmed:volume
389
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
622-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Property of cold inducible DEAD-box RNA helicase in hyperthermophilic archaea.
pubmed:affiliation
Department of Bioscience, Graduate School of Science and Technology, Kwansei-Gakuin University, 2-1 Gakuen, Sanda, Hyogo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't