Linked Life Data

1974892

Source:http://linkedlifedata.com/resource/pubmed/id/1974892

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1990-9-25
pubmed:abstractText
Retinal degeneration is inherited in an autosomal recessive pattern in the retinal degeneration (rd) mouse. The defective gene for this disease has been mapped to mouse chromosome 5 between the well-defined (anchor) genes Afp and Gus. We recently cloned a putative rd cDNA, zr.408, using a strategy based on subtractive and differential hybridization. zr.408 was shown to hybridize to a larger message from rd/rd mice than from normal mice in Northern blots; was mapped to mouse chromosome 5; and was used to detect restriction fragment length polymorphisms (RFLPs) between rd/rd and +/+ DNA in genomic Southern blots. In order to obtain further evidence that zr.408 does in fact correspond to the rd gene, we used two methods to position zr.408 on chromosome 5. Analysis of an intersubspecific backcross localized the sequences corresponding to zr.408 between the genes Afp and Gus, as expected for rd. The second approach involved an interspecific backcross using C57BL/6J-rd/rd mice congenic for the normal allele of rd, which was derived from the wild mouse, Mus spretus; the results of this study showed that zr.408 is at or near the rd locus. These two studies add evidence to the existing data, which suggest that zr.408 is the correspondent of the rd gene.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0146-0404
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1427-32
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Fine mapping of a putative rd cDNA and its co-segregation with rd expression.
pubmed:affiliation
Loyola Marymount University, Los Angeles, California.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't