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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
1990-9-21
|
| pubmed:abstractText |
We have examined the importance of L3T4+ (murine equivalent to CD4+) cells for hematopoietic regulation in vivo in unperturbed mice and mice recovering from total-body irradiation (TBI) using a cytotoxic monoclonal antibody (MoAb) raised with the GK 1.5 hybridoma. Ablating L3T4+ cells in "normal" (unperturbed) B6D2F1 mice substantially decreased the S-phase fraction (determined by in vivo hydroxyurea suicide) of erythroid progenitor cells (erythroid colony-forming units, CFU-E) as compared to the pretreatment level (10% +/- 14.1% [day 3 following depletion] vs 79.8% +/- 15.9%, respectively) with a corresponding decrease in the marrow content of CFU-E at this time to approximately 1% of the pretreatment value. Although the S-phase fraction of CFU-GM was decreased to 2.2% +/- 3.1% 3 days after L3T4+ cell ablation from the 21.3% +/- 8.3% pretreatment value, CFU-GM cellularity showed little change over the 3 days following anti-L3T4 treatment. Anti-L3T4 MoAb treatment had little or no effect on either the S-phase fraction or the marrow content of hematopoietic "stem cells" (spleen colony-forming units, CFU-S) committed to myeloerythroid differentiation. Ablating L3T4+ cells prior to a single dose of 2 Gy TBI resulted in significantly reduced marrow contents of CFU-S on day 3 and granulocyte-macrophage colony-forming units (CFU-GM) on day 6 following TBI, with little or no effect on the corresponding recovery of CFU-E. The present findings provide the first in vivo evidence that L3T4+ cells are involved in: 1) maintaining the proliferative activity of CFU-E and CFU-GM in unperturbed mice and 2) supporting the restoration of CFU-S and CFU-GM following TBI-induced myelosuppression.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0301-472X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
18
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
863-7
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:1974863-Animals,
pubmed-meshheading:1974863-Antibodies, Monoclonal,
pubmed-meshheading:1974863-Bone Marrow,
pubmed-meshheading:1974863-Bone Marrow Cells,
pubmed-meshheading:1974863-CD4-Positive T-Lymphocytes,
pubmed-meshheading:1974863-Cell Count,
pubmed-meshheading:1974863-Cytotoxicity, Immunologic,
pubmed-meshheading:1974863-Erythroid Precursor Cells,
pubmed-meshheading:1974863-Granulocytes,
pubmed-meshheading:1974863-Hematopoiesis,
pubmed-meshheading:1974863-Hematopoietic Stem Cells,
pubmed-meshheading:1974863-Macrophages,
pubmed-meshheading:1974863-Male,
pubmed-meshheading:1974863-Mice,
pubmed-meshheading:1974863-Mice, Inbred C57BL,
pubmed-meshheading:1974863-Mice, Inbred DBA,
pubmed-meshheading:1974863-Spleen,
pubmed-meshheading:1974863-Whole-Body Irradiation
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Differential effect of L3T4+ cells on recovery from total-body irradiation.
|
| pubmed:affiliation |
Wayne State University School of Medicine, Department of Internal Medicine, Detroit, MI 48201.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|