Linked Life Data

19748476

Source:http://linkedlifedata.com/resource/pubmed/id/19748476

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-11-27
pubmed:abstractText
Based on the biosynthetic pathway of firefly bioluminescence substrate D-luciferin, the concentration of L-cysteine can be quantified using a simple protocol and a conventional luminescence detector. The lower limit of quantification (signal/noise ratio [S/N]=10) was 0.26 microM. Using our method, the total amount of free/reduced and disulfide/oxidized L-cysteine could be measured successfully in human serum. In addition, biosynthetic precursors such as 2-cyano-6-hydroxybenzothiazole and L-luciferin could replace d-luciferin in the cell-based luciferase assay. Our results suggest that the bioluminescence reaction associated with the biosynthesis of bioluminescence substrates can provide a fast and cost-effective assay method.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1096-0309
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
396
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
316-8
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Applications of luciferin biosynthesis: Bioluminescence assays for l-cysteine and luciferase.
pubmed:affiliation
National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8568, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Technical Report