Source:http://linkedlifedata.com/resource/pubmed/id/19746735
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
16
|
| pubmed:dateCreated |
2009-9-14
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| pubmed:abstractText |
A three-spined stickleback (Gasterosteus aculeatus) cDNA array and one-dimensional 1H nuclear magnetic resonance (NMR) spectroscopy-based metabolomics approach, together with individual biomarkers,were employed to investigate the responses of male sticklebacks to polycyclic aromatic hydrocarbon exposure. Fish were exposed to 1,2:5,6-dibenzanthracene (DbA) at concentrations between 0.01 and 50 microg per liter dissolved in the ambient water for four days, and hepatic transcript and metabolite profiles were determined in comparison with those of solvent-exposed controls. Induction of gene expression was apparent for cytochrome P450 1A (CYP1A) and CYP2-family monooxygenases and these responses were strongly correlated with DbA exposure concentrations (for CYP1A r > 0.996). Expression of suites of genes related to bile acid biosynthesis, steroid metabolism, and endocrine function were also affected, as demonstrated by gene ontology analyses. Expression changes in selected genes were confirmed by real-time PCR. Metabolomics highlighted notable changes in concentrations of taurine, malonate, glutamate, and alanine. These statistically significant responses to environmentally relevant concentrations of DbA at the transcriptomic and metabolomic levels provided sensitive markers characteristic of environmentally relevant low-level DbA exposure. Metabolic pathways were identified where both gene expression and metabolite concentrations were altered in response to DbA.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0013-936X
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| pubmed:author |
pubmed-author:BakerPaulP,
pubmed-author:BallJonJ,
pubmed-author:BrownMargaret MMM,
pubmed-author:ChipmanJames KJK,
pubmed-author:CraftJohn AJA,
pubmed-author:FalcianiFrancescoF,
pubmed-author:KatsiadakiIoannaI,
pubmed-author:OrtegaFernandoF,
pubmed-author:SantosEduarda MEM,
pubmed-author:TylerCharles RCR,
pubmed-author:ViantMark RMR,
pubmed-author:WilliamsTim DTD,
pubmed-author:WuHuifengH
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| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
43
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
6341-8
|
| pubmed:meshHeading |
pubmed-meshheading:19746735-Animals,
pubmed-meshheading:19746735-Benz(a)Anthracenes,
pubmed-meshheading:19746735-Endocrine System,
pubmed-meshheading:19746735-Environmental Exposure,
pubmed-meshheading:19746735-Environmental Monitoring,
pubmed-meshheading:19746735-Gene Expression Profiling,
pubmed-meshheading:19746735-Gene Expression Regulation,
pubmed-meshheading:19746735-Liver,
pubmed-meshheading:19746735-Male,
pubmed-meshheading:19746735-Metabolic Detoxication, Phase I,
pubmed-meshheading:19746735-Metabolic Networks and Pathways,
pubmed-meshheading:19746735-Metabolomics,
pubmed-meshheading:19746735-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:19746735-Principal Component Analysis,
pubmed-meshheading:19746735-Smegmamorpha,
pubmed-meshheading:19746735-Steroids,
pubmed-meshheading:19746735-Toxicity Tests
|
| pubmed:year |
2009
|
| pubmed:articleTitle |
Hepatic transcriptomic and metabolomic responses in the stickleback (Gasterosteus aculeatus) exposed to environmentally relevant concentrations of dibenzanthracene.
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| pubmed:affiliation |
School of Biosciences, The University of Birmingham, Birmingham, B15 2TT, UK. t.d.williams@bham.ac.uk
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|