Linked Life Data

1974577

Source:http://linkedlifedata.com/resource/pubmed/id/1974577

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-9-14
pubmed:abstractText
To determine the radiosensitivity of non-S-phase tumour cells in vitro, survival curves of SCC VII tumour cells were obtained after a short block with hydroxyurea. Dose-response curves of micronucleus (MN) frequency appearing in non-S-phase cells were also determined by excluding S-phase cells with immunofluorescence staining to 5-bromo-2'-deoxyuridine (BUdR). Both the dose-response curves of MN frequency and survival curves were analysed by a linear-quadratic model (surviving fraction = exp (-alpha D-beta D2), MN frequency = aD+bD2+c). A good correlation between the alpha/beta and alpha/beta ratios was observed. In both BUdR-unincorporated and asynchronous cell cultures, the regression lines between the surviving fraction and micronucleus frequency were statistically identical. Therefore, it was shown that cell survival curves, which cannot be obtained directly by the routine colony-formation technique, can be calculated using the micronucleus frequency and the regression line in asynchronous cell cultures. Therefore, it should be possible to detect the response to irradiation of quiescent cells in tumours using the immunofluorescence staining to BUdR and the MN frequency assay.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0955-3002
pubmed:author
pubmed:issnType
Print
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
303-11
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Use of the micronucleus assay for the selective detection of radiosensitivity in BUdR-unincorporated cells after pulse-labelling of exponentially growing tumour cells.
pubmed:affiliation
Department of Radiology, Faculty of Medicine, Kyoto University, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't