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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1990-8-31
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pubmed:abstractText |
The electrophysiological and pharmacological properties of N-methyl-D-aspartate (NMDA)-sensitive receptors expressed in Xenopus oocytes by injection of total poly(A)+RNAs (mRNAs) from the cerebellum and cerebrum of guinea pigs were compared. The inward current induced by NMDA under voltage-clamp in cerebellar mRNA-injected oocytes was depressed in a voltage-dependent fashion by Mg2+ to show a negative slope conductance and selectively antagonized by D-2-amino-5-phosphonovalerate (D-APV) and phencyclidine (PCP). Glycine (0.01-10 microM) did not potentiate NMDA-induced currents in cerebellar mRNA-injected oocytes, while it potentiated NMDA-induced currents in cerebral mRNA-injected oocytes in a dose-dependent fashion. 6-Cyano-7-nitroquinoxaline-2,3-dione and 7-chlorokynure-nate suppressed the NMDA response but significantly less potently in cerebellar mRNA-injected oocytes than in cerebral mRNA-injected oocytes. These results suggest that the NMDA-sensitive receptor expressed in Xenopus oocytes by guinea pig cerebellar mRNA resembles the cerebral NMDA receptor in its high sensitivities to Mg2+, PCP, and D-APV, but it is distinct from the cerebral NMDA receptor in responsiveness to glycine.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-Amino-5-phosphonovalerate,
http://linkedlifedata.com/resource/pubmed/chemical/6-Cyano-7-nitroquinoxaline-2,3-dione,
http://linkedlifedata.com/resource/pubmed/chemical/7-chlorokynurenic acid,
http://linkedlifedata.com/resource/pubmed/chemical/Aspartic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Glycine,
http://linkedlifedata.com/resource/pubmed/chemical/Kynurenic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Magnesium,
http://linkedlifedata.com/resource/pubmed/chemical/N-Methylaspartate,
http://linkedlifedata.com/resource/pubmed/chemical/Phencyclidine,
http://linkedlifedata.com/resource/pubmed/chemical/Quinoxalines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, N-Methyl-D-Aspartate,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Neurotransmitter
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0270-6474
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2148-55
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:1973946-2-Amino-5-phosphonovalerate,
pubmed-meshheading:1973946-6-Cyano-7-nitroquinoxaline-2,3-dione,
pubmed-meshheading:1973946-Animals,
pubmed-meshheading:1973946-Aspartic Acid,
pubmed-meshheading:1973946-Cerebellum,
pubmed-meshheading:1973946-Electric Conductivity,
pubmed-meshheading:1973946-Female,
pubmed-meshheading:1973946-Glycine,
pubmed-meshheading:1973946-Guinea Pigs,
pubmed-meshheading:1973946-Kynurenic Acid,
pubmed-meshheading:1973946-Magnesium,
pubmed-meshheading:1973946-Male,
pubmed-meshheading:1973946-Membrane Potentials,
pubmed-meshheading:1973946-N-Methylaspartate,
pubmed-meshheading:1973946-Oocytes,
pubmed-meshheading:1973946-Phencyclidine,
pubmed-meshheading:1973946-Quinoxalines,
pubmed-meshheading:1973946-RNA, Messenger,
pubmed-meshheading:1973946-Receptors, N-Methyl-D-Aspartate,
pubmed-meshheading:1973946-Receptors, Neurotransmitter,
pubmed-meshheading:1973946-Xenopus laevis
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pubmed:year |
1990
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pubmed:articleTitle |
Glycine-insensitive NMDA-sensitive receptor expressed in Xenopus oocytes by guinea pig cerebellar mRNA.
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pubmed:affiliation |
Department of Pharmacology, National Defense Medical College, Saitama, Japan.
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pubmed:publicationType |
Journal Article
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