Linked Life Data

19736561

Source:http://linkedlifedata.com/resource/pubmed/id/19736561

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2009-9-18
pubmed:abstractText
Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a technique for genome-wide profiling of DNA-binding proteins, histone modifications or nucleosomes. Owing to the tremendous progress in next-generation sequencing technology, ChIP-seq offers higher resolution, less noise and greater coverage than its array-based predecessor ChIP-chip. With the decreasing cost of sequencing, ChIP-seq has become an indispensable tool for studying gene regulation and epigenetic mechanisms. In this Review, I describe the benefits and challenges in harnessing this technique with an emphasis on issues related to experimental design and data analysis. ChIP-seq experiments generate large quantities of data, and effective computational analysis will be crucial for uncovering biological mechanisms.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1471-0064
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
669-80
pubmed:dateRevised
2011-10-12
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
ChIP-seq: advantages and challenges of a maturing technology.
pubmed:affiliation
Harvard Medical School, 10 Shattuck Street, Boston, MA 02115, USA. peter_park@harvard.edu
pubmed:publicationType
Journal Article, Review, Research Support, N.I.H., Extramural