19735482

Source:http://linkedlifedata.com/resource/pubmed/id/19735482

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0004083, umls-concept:C0017471, umls-concept:C0024554, umls-concept:C0033684, umls-concept:C0475264, umls-concept:C0591833, umls-concept:C1418598, umls-concept:C1420677, umls-concept:C1511545
pubmed:issue	10
pubmed:dateCreated	2009-9-25
pubmed:abstractText	The mouse Piwi family proteins (MILI, MIWI and MIWI2) play pivotal roles in spermatogenesis through transcriptional and post-transcriptional gene regulation. To reveal the molecular functions of these proteins, we investigate the proteins that bind to MILI in adult mouse testes. We found that both MILI and MIWI bind to TDRD1/MTR-1, which is also an essential protein for spermatogenesis. Co-immunoprecipitation assays and subcellular localization of the proteins and mutants thereof revealed a complex formation involving MILI, MIWI and TDRD1/MTR-1. In addition, the subcellular localizations of MILI and TDRD1/MTR-1 were altered, and chromatoid body formation was impaired in the MIWI-null round spermatids. These data suggest that the formation of complexes between MILI, MIWI and TDRD1/MTR-1 is critical for the integrated subcellular localizations of these proteins, and is presumably essential for spermatogenesis.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/9607379
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Argonaute Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Piwil1 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins, Small Nuclear, http://linkedlifedata.com/resource/pubmed/chemical/Tdrd1 protein, mouse
pubmed:status	MEDLINE
pubmed:month	Oct
pubmed:issn	1365-2443
pubmed:author	pubmed-author:ChumaShinichiroS, pubmed-author:KimuraTohruT, pubmed-author:KojimaKanakoK, pubmed-author:Kuramochi-MiyagawaSatomiS, pubmed-author:NakanoToruT, pubmed-author:NakatsujiNorioN, pubmed-author:TanakaTakashiT
pubmed:issnType	Electronic
pubmed:volume	14
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	1155-65
pubmed:dateRevised	2011-11-17
pubmed:meshHeading	pubmed-meshheading:19735482-Animals, pubmed-meshheading:19735482-Argonaute Proteins, pubmed-meshheading:19735482-Blotting, Western, pubmed-meshheading:19735482-Cell Line, pubmed-meshheading:19735482-Cell Nucleus, pubmed-meshheading:19735482-Cytoplasm, pubmed-meshheading:19735482-Green Fluorescent Proteins, pubmed-meshheading:19735482-Humans, pubmed-meshheading:19735482-Immunohistochemistry, pubmed-meshheading:19735482-Immunoprecipitation, pubmed-meshheading:19735482-Male, pubmed-meshheading:19735482-Mice, pubmed-meshheading:19735482-Mice, Inbred C57BL, pubmed-meshheading:19735482-Mice, Knockout, pubmed-meshheading:19735482-Microscopy, Electron, pubmed-meshheading:19735482-Mutation, pubmed-meshheading:19735482-Protein Binding, pubmed-meshheading:19735482-Proteins, pubmed-meshheading:19735482-Recombinant Fusion Proteins, pubmed-meshheading:19735482-Ribonucleoproteins, Small Nuclear, pubmed-meshheading:19735482-Spermatocytes, pubmed-meshheading:19735482-Testis, pubmed-meshheading:19735482-Transfection
pubmed:year	2009
pubmed:articleTitle	Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells.
pubmed:affiliation	Department of Pathology, Medical School, Osaka University, Osaka 565-0871, Japan.
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't