Linked Life Data

19733560

Source:http://linkedlifedata.com/resource/pubmed/id/19733560

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-10-13
pubmed:abstractText
Inflammation plays a central role in the development of numerous disorders of the central nervous system (CNS) such as multiple sclerosis (MS). For a long time it was assumed that recruitment of macrophages into the CNS and breakdown of the blood-brain barrier (BBB) are closely linked. In the present study we challenge this concept. We used small superparamagnetic iron oxide particles (SPIO)-enhanced T2-weighted (T2-w) magnetic resonance imaging (MRI) on a routine 1.5 T MRI unit to follow macrophage infiltration in experimental autoimmune encephalomyelitis (EAE), the animal model of MS. After an initial SPIO-enhanced MRI, gadofluorine M (Gf), an experimental contrast agent which proved to be more sensitive in detecting BBB leakage than gadolinium (Gd)-DTPA (Bendszus, M., Ladewig, G., Jestaedt, L., Misselwitz, B., Solymosi, L., Toyka, K.V., Stoll, G., Gadofluorine-M enhancement allows more sensitive detection of inflammatory CNS lesions than T2-w imaging: a quantitative MRI study. Brain 2008; 1-12), was applied to the same animals followed by a second scan. Areas with SPIO-induced signal loss on T2-w MRI indicative of recent macrophage infiltration were matched with areas showing Gf enhancement as a measure of BBB disturbance. Overall 87 EAE lesions showed iron-related signal loss, while 57 lesions showed Gf enhancement. By direct comparison we could detect concomitant SPIO-induced signal loss and Gf enhancement only in a small minority of lesions. In conclusion, our findings show macrophage infiltration in the CNS during EAE in areas with a closed BBB for humoral factors. This holds true despite the use of a more sensitive MR contrast agent for BBB disruption than Gd-DTPA. Our experimental observations may have implications for disease monitoring in MS patients by MRI which guides treatment decisions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1090-2430
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
220
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
207-11
pubmed:meshHeading
pubmed-meshheading:19733560-Animals, pubmed-meshheading:19733560-Blood-Brain Barrier, pubmed-meshheading:19733560-Chemotaxis, Leukocyte, pubmed-meshheading:19733560-Contrast Media, pubmed-meshheading:19733560-Disease Models, Animal, pubmed-meshheading:19733560-Disease Progression, pubmed-meshheading:19733560-Encephalitis, pubmed-meshheading:19733560-Encephalomyelitis, Autoimmune, Experimental, pubmed-meshheading:19733560-Female, pubmed-meshheading:19733560-Ferric Compounds, pubmed-meshheading:19733560-Macrophages, pubmed-meshheading:19733560-Magnetic Resonance Imaging, pubmed-meshheading:19733560-Multiple Sclerosis, pubmed-meshheading:19733560-Organometallic Compounds, pubmed-meshheading:19733560-Predictive Value of Tests, pubmed-meshheading:19733560-Rats, pubmed-meshheading:19733560-Sensitivity and Specificity
pubmed:year
2009
pubmed:articleTitle
Spatial diversity of blood-brain barrier alteration and macrophage invasion in experimental autoimmune encephalomyelitis: a comparative MRI study.
pubmed:affiliation
Department of Neurology, University of Würzburg, Josef-Schneider-Str. 11, D-97080 Würzburg, Germany.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't