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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2009-9-4
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pubmed:abstractText |
Adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) has been successfully used to treat patients with different types of cancer. However, the long-term spatial-temporal dynamics of the distribution of systemically infused CTLs remains largely unknown. Noninvasive imaging of adoptively transferred CTLs using molecular-genetic reporter imaging with positron emission tomography and computed tomography (PET-CT) represents an innovative approach to understanding the long-term migratory patterns and therapeutic potential of adoptively transferred T cells. Here we report the application of repetitive PET-CT imaging with [18F]fluoro-5-ethyl-1-beta-D-arabinofuranosyluracil (18F-FEAU) in two nonhuman primates demonstrating that autologous polyclonal macaque T lymphocytes activated and transduced with a retroviral vector encoding for the sr39 mutant herpes simplex virus 1 thymidine kinase (sr39HSV1-tk) reporter gene can be detected after intravenous infusion in discrete lymphoid organs and in sites of inflammation. This study represents a proof of principle and supports the application of 18F-FEAU PET-CT imaging for monitoring the distribution of intravenously administered sr39HSV1-tk gene-transduced CTLs in humans.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1535-3508
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pubmed:author |
pubmed-author:AlauddinMianM,
pubmed-author:BollardCatherine MCM,
pubmed-author:BorneAgathaA,
pubmed-author:BrammerDavidD,
pubmed-author:CooperLaurence J NLJ,
pubmed-author:DeckerWilliam KWK,
pubmed-author:DottiGianpietroG,
pubmed-author:GelovaniJuri GJG,
pubmed-author:GonzalezCarlosC,
pubmed-author:JacksonJamesJ,
pubmed-author:KornblauStevenS,
pubmed-author:MariniFrankF,
pubmed-author:MawlawiOsamaO,
pubmed-author:NajjarAmerA,
pubmed-author:PaolilloVincenzoV,
pubmed-author:SavoldoBarbaraB,
pubmed-author:ShpallElizabeth JEJ,
pubmed-author:SmithAmandaA,
pubmed-author:SteinerDavidD,
pubmed-author:TianMeiM,
pubmed-author:UthamanthilRajeshR
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
230-7
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:19728977-Adoptive Transfer,
pubmed-meshheading:19728977-Animals,
pubmed-meshheading:19728977-Arabinofuranosyluracil,
pubmed-meshheading:19728977-Cells, Cultured,
pubmed-meshheading:19728977-Female,
pubmed-meshheading:19728977-Fluorine Radioisotopes,
pubmed-meshheading:19728977-Genes, Reporter,
pubmed-meshheading:19728977-Herpesvirus 1, Human,
pubmed-meshheading:19728977-Infusions, Intravenous,
pubmed-meshheading:19728977-Macaca mulatta,
pubmed-meshheading:19728977-Male,
pubmed-meshheading:19728977-Monitoring, Immunologic,
pubmed-meshheading:19728977-Positron-Emission Tomography,
pubmed-meshheading:19728977-Primates,
pubmed-meshheading:19728977-T-Lymphocytes,
pubmed-meshheading:19728977-Thymidine Kinase,
pubmed-meshheading:19728977-Tomography, X-Ray Computed
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pubmed:articleTitle |
Repetitive noninvasive monitoring of HSV1-tk-expressing T cells intravenously infused into nonhuman primates using positron emission tomography and computed tomography with 18F-FEAU.
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pubmed:affiliation |
Department of Experimental Diagnostic Imaging, The University of Texas, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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