Linked Life Data

19726177

Source:http://linkedlifedata.com/resource/pubmed/id/19726177

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-9-15
pubmed:abstractText
In this paper, a very simple and easily-operated colorimetric multiplexed immunoassay method for sequential detection of tumor markers has been presented. Magnetic microparticles which are conjugated with biotinylated antibodies are firstly added into the test solution. After fast magnetic collection, these complexes are separated from non-specific proteins. Through different enzymatic reactions of 3,3',5,5'-tetramethylbenzidine (TMB) and o-phenylenediamine (OPD) catalyzed by horseradish peroxidase molecules which are loaded on the surfaces of gold nanoparticles, two antigens carcinoembryonic antigen and alpha-fetoprotein can be detected even with naked eyes. The detection limit obtained from the spectrophotometric measurements is as low as 0.02 ng/mL. This proposed method also has high specificity and reproducibility, as well as excellent efficiency of 94 min for the detection of serum samples. So, this new multiplexed immunoassay method might be a promising approach for the diagnosis of cancer and some other diseases in clinical applications.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1873-4235
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
532-6
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Colorimetric multiplexed immunoassay for sequential detection of tumor markers.
pubmed:affiliation
Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 210093 Nanjing, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies