Linked Life Data

19724854

Source:http://linkedlifedata.com/resource/pubmed/id/19724854

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-9-2
pubmed:abstractText
Radiosensitization of cancer cells to irradiation could improve the efficacy of radiotherapy. The early transcriptional factor (Egr-1) promoter induced expression of downstream genes after irradiation. TNF-related apoptosis-inducing ligand (TRAIL) is known to induce apoptosis in malignant cells, but displayed little or no toxicity on normal cells. In this study, we constructed pcDNA3.1-Egr-1-TRAIL (pEgr.1-TRAIL) recombinant plasmid and evaluated its effect on human colon cancer cell line SW480. pEgr.1-TRAIL transfection combined with radiotherapy caused dramatically elevation of TRAIL expression both in mRNA and protein levels, much lower radiobiological parameters in clonogenic assays, accompanied by remarkably increase in apoptosis ratio. Furthermore, pEgr.1-TRAIL transfected cells displayed higher proportion in G0/G1 phase. Our results suggested that pEgr.1-TRAIL can sensitize SW480 cells to radiation, and the radiosensitization is related to cell cycle changes and apoptosis mediated by up-regulation of TRAIL expression. These findings support the potential future application of genetic radiotherapy against carcinoma.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1021-335X
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
765-71
pubmed:meshHeading
pubmed-meshheading:19724854-Apoptosis, pubmed-meshheading:19724854-Blotting, Western, pubmed-meshheading:19724854-Cell Line, Tumor, pubmed-meshheading:19724854-Colonic Neoplasms, pubmed-meshheading:19724854-Early Growth Response Protein 1, pubmed-meshheading:19724854-Gene Expression Regulation, Neoplastic, pubmed-meshheading:19724854-Gene Therapy, pubmed-meshheading:19724854-Humans, pubmed-meshheading:19724854-Plasmids, pubmed-meshheading:19724854-Promoter Regions, Genetic, pubmed-meshheading:19724854-Radiation Tolerance, pubmed-meshheading:19724854-Recombinant Proteins, pubmed-meshheading:19724854-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:19724854-TNF-Related Apoptosis-Inducing Ligand, pubmed-meshheading:19724854-Transfection, pubmed-meshheading:19724854-Up-Regulation
pubmed:year
2009
pubmed:articleTitle
Enhanced radiosensitivity of SW480 cells via TRAIL up-regulation mediated by Egr-1 promoter.
pubmed:affiliation
Department of Radiation and Medical Oncology, Wuhan University, Wuhan, 430071, P.R. China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't